Molecular and Cellular Biochemistry

, Volume 20, Issue 2, pp 111–118 | Cite as

On the heterogeneity of the slow reassociating (“unique”) DNA

  • Ivan G. Ivanov
  • George G. Markov
Article
Received:

Summary

The slow reassociating fraction of mouse DNA (“unique DNA”), when allowed to reassociate in 0.14 m sodium phosphate buffer at 50 °C showed a biphasic melting curve with a transition at 78–80 °C. On the basis of this feature, the slow reassociating DNA was separated preparatively into two fractions: “unique DNA” I and II. Their duplexes showed differences with respect to thermal stability, S1 nuclease resistance and rate of reassociation. About one third of the sequences in each fraction were fraction-specific. The conclusion was drawn that for “unique DNA” I these should be the low repetitive or single copy related sequences (multigene families) and for “unique DNA” II—the unrelated single copy sequences or recent families of low repetitive not yet diverged sequences.

Keywords

Sodium Phosphate Phosphate Buffer Thermal Stability Sodium Phosphate 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.
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Copyright information

© Dr. W. Junk b. v. Publishers 1978

Authors and Affiliations

  • Ivan G. Ivanov
    • 1
  • George G. Markov
    • 1
  1. 1.Institute of BiochemistryBulgarian Academy of SciencesSofiaBulgaria

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