Cell and Tissue Research

, Volume 207, Issue 1, pp 21–29 | Cite as

On the origin of Z-band material and myofilaments in myoblasts from the human atrial wall

  • Thorvald Sætersdal
  • Hogne Engedal
  • Reidar Lie
  • Reidar Myklebust
Article
Accepted:

Summary

The origin of cardiac myofibrils in cells from the atrial wall in human embryos was studied. Z-band substance appears throughout the cytoplasm as irregular electron dense patches in a network of thin filaments. The thin and thick filaments are synthesized as separate units in the sarcoplasm and are later aggregated into myofibrils. Complexes of Z substance and thin filaments occur numerously at different stages of myofibrillar organisation. Thick filaments are formed in close proximity to free ribosomes and are later incorporated in an hexagonal pattern into the Z-band/thin filament complex.

Key words

Human heart Cardiac ultrastructure Sarcomerogenesis Protein synthesis 
This is a preview of subscription content, log in to check access.

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. Allen ER, Pepe RA (1965) Ultrastructure of developing muscle cells in the chick embryo. Am J Anat 116:115–147Google Scholar
  2. Bishop SP, Cole CR (1969) Ultrastructural changes in the canine myocardium with right ventricular hypertrophy and congestive heart failure. Lab Invest 20:219–229Google Scholar
  3. Cedergren B, Harary I (1964) In vitro studies on single beating rat heart cells. VI. Electron microscopic studies of single cells. J Ultrastruct Res 11:428–442Google Scholar
  4. Fischman DA (1967) An electron microscope study of myofibril formation in embryonic chick skeletal muscle. J Cell Biol 32:557–575Google Scholar
  5. Grimley PM, Edwards GA (1960) The ultrastructure of cardiac desmosomes in the toad and their relationship to the intercalated disc. J Biophys Biochem Cytol 8:305–318Google Scholar
  6. Hagopian M, Spiro D (1970) Derivation of the Z line in the embryonic chick heart. J Cell Biol 44:683–687Google Scholar
  7. Hay ED (1963) The fine structure of differentiating muscle in the salamander tail. Z Zellforsch 59:6–34Google Scholar
  8. Hibbs RG (1956) Electron microscopy of developing cardiac muscle in chick embryos. Am J Anat 99:17–51Google Scholar
  9. Huang CY (1967) Electron microscopic study of the development of heart muscle of the frog, Rana pipiens. J Ultrastruct Res 20:211–226Google Scholar
  10. Karnovsky MJ (1965) A formaldehyde-glutaraldehyde fixative of high osmolality for use in electron microscopy. J Cell Biol 27:137AGoogle Scholar
  11. Legato MJ (1970) Sarcomerogenesis in human myocardium. J Mol Cell Cardiol 1:425–437Google Scholar
  12. Legato MJ (1972) Ultrastructural characteristics of the rat ventricular cell grown in tissue culture, with special reference to sarcomerogenesis. J Mol Cell Cardiol 4:299–317Google Scholar
  13. Legato MJ (1973) The role of Z substance in sarcomerogenesis. J Mol Cell Cardiol 5:205–206Google Scholar
  14. Manesek FJ (1968) Embryonic development of the heart. I. A light and electron microscopic study of myocardial development in the early chick embryo. J Morphol 125:329–366Google Scholar
  15. Markwald RR (1973) Distribution and relationship of precursor Z material to organizing myofibrillar bundles in embryonic rat and hamster ventricular myocytes. J Mol Cell Cardiol 5:341–350Google Scholar
  16. Maron BJ, Ferrans VJ (1978) Ultrastructural features of hypertrophied human ventricular myocardium. Progr Cardiovasc Diseases 21:207–238Google Scholar
  17. Myklebust R, Sætersdal TS, Engedal H, Ulstein M, Ødegården S (1978) Ultrastructural studies on the formation of myofilaments and myofibrils in the human embryonic and adult hypertrophied heart. Anat Embryol 152:127–140Google Scholar
  18. Reynolds ES (1963) The use of lead citrate as an electron dense stain in electron microscopy. J Cell Biol 17:208CrossRefPubMedGoogle Scholar
  19. Rønnau KC (1977) Myogenesis and contraction in the early embryonic heart of the rainbow trout. Cell Tissue Res 180:123–132PubMedGoogle Scholar
  20. Sætersdal TS, Myklebust R, Skagseth E, Engedal H (1976) Ultrastructural studies on the growth of filaments and sarcomeres in mechanically overloaded human hearts. Virchows Arch B Cell Pathol 21:91–112Google Scholar
  21. Schiebler TH, Wolff HH (1966) Elektronenmikroskopische Untersuchungen am Herzmuskel der Ratte während der Entwicklung. Z Zellforsch 69:22–40Google Scholar
  22. Sommer JR, Johnson EA (1979) Ultrastructure of cardiac muscle. In: Handbook of physiology. The cardiovascular system. I. pp 113–186Google Scholar
  23. Wainrach S, Sotelo JR (1961) Electron microscope study of the developing chick embryo heart. Z Zellforsch 55:622–634Google Scholar

Copyright information

© Springer-Verlag 1980

Authors and Affiliations

  • Thorvald Sætersdal
    • 1
    • 2
  • Hogne Engedal
    • 1
    • 2
  • Reidar Lie
    • 1
    • 2
  • Reidar Myklebust
    • 1
    • 2
  1. 1.Cellular Cardiology Research GroupInstitute of Anatomy, University of BergenBergenNorway
  2. 2.Division of Cardiac SurgeryBergen University HospitalBergenNorway

Personalised recommendations