Plant Cell Reports

, Volume 11, Issue 12, pp 597–600 | Cite as

Characteristics of growth and tropane alkaloid production in Hyoscyamus albus hairy roots transformed with Agrobacterium rhizogenes A4

  • Philippe Christen
  • Toshio Aoki
  • Koichiro Shimomura


Hairy root culture of Hyoscyamus albus was established by transformation with Agrobacterium rhizogenes strain A4. The growth and production of five tropane alkaloids were investigated under various culture conditions. Among the four basal culture media tested, Woody Plant medium was the best for growth of the hairy roots, but a high amount of tropane alkaloids was obtained with Gamborg's B5 medium. Sucrose concentration in B5 medium had little effect on the growth, while 3% sucrose was suitable for the alkaloid production. Addition of KNO3 to Woody Plant medium affected the growth, whereas the alkaloid content was not markedly improved. Supplement of some metal ions to B5 medium stimulated the alkaloid production. In particular, Cu2+ remarkably enhanced both the growth and the alkaloid yield. The hairy roots cultured under 16 h/day light survived for more than 32 days compared with those cultured in the dark.

Key words

Hyoscyamus albus Agrobacterium rhizogenes Hairy root culture Tropane alkaloids 



ethylenediaminetetraacetic acid


high performance liquid chromatography



MS medium

Murashige and Skoog medium

WP medium

McCown's Woody Plant medium

B5 medium

Gamborg B5 medium




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  1. Christen P, Roberts MF, Phillipson JD, Evans WC (1989) Plant Cell Reports 8: 75–77Google Scholar
  2. Fujita Y, Hara Y, Suga C, Morimoto T (1981) Plant Cell Reports 1: 61–63Google Scholar
  3. Gamborg OL, Miller RA, Ojima K (1968) Exp. Cell. Res. 50: 151–158Google Scholar
  4. Gränicher F, Christen P, Kapétanidis I (1992) Plant Cell Reports 11:339–342Google Scholar
  5. Ishimaru K, Shimomura K (1989) Phytochemistry 28: 3507–3510Google Scholar
  6. Jung G, Tepfer D (1987) Plant Science 50: 145–151Google Scholar
  7. Kamada H, Okamura N, Satake M, Harada H, Shimomura K (1986) Plant Cell Reports 5: 239–242Google Scholar
  8. Lloyd GB, McCown BH (1980) Inter. Plant Prop. Soc. 30: 421–427Google Scholar
  9. Murashige T, Skoog F (1962) Physiol. Plant. 15: 473–497Google Scholar
  10. Petit A, David C, Dahl GA, Ellis JG, Guyon P, Casse-Delbart F, Tempé J (1983) Mol. Gen. Genet. 190: 204–214Google Scholar
  11. Sauerwein M, Ishimaru K, Shimomura K (1991) Phytochemistry 30: 2977–2978Google Scholar
  12. Sauerwein M, Shimomura K (1991) Phytochemistry 30: 3277–3280Google Scholar
  13. Schmidt GC, Eling TE, Drach JC (1967) J. Pharm. Sci. 56: 215–221Google Scholar
  14. Shimomura K, Sauerwein M, Ishimaru K (1991) Phytochemistry 30: 2275–2278CrossRefGoogle Scholar
  15. Tepfer D (1990) Physiol. Plant. 79: 140–146Google Scholar
  16. Vervliet G, Holsters M, Teuchy H, Montagu M van, Schell J (1975) J. Gen. Virol. 26: 33–48Google Scholar

Copyright information

© Springer-Verlag 1992

Authors and Affiliations

  • Philippe Christen
    • 1
  • Toshio Aoki
    • 1
  • Koichiro Shimomura
    • 1
  1. 1.Tsukuba Medicinal Plant Research Station, National Institute of Hygienic SciencesIbarakiJapan

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