Plant Cell Reports

, Volume 13, Issue 2, pp 119–122 | Cite as

Introduction of mRNA to plant protoplasts using polyethylene glycol

  • Daniel R. Gallie
Article
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Revised:

Abstract

A method is described to deliver mRNA to plant protoplasts using polyethylene glycol for transient expression. In order to optimize delivery and subsequent expression, we have examined a number of parameters that affect expression levels including the requirement for Mg2+ and Ca2+ ions, carrier nucleic acid, and the linearity of the dose-response. Delivery of mRNA using PEG results in levels of expression that are comparable to those obtained with electroporation. This method is particularly useful for those species in which electroporation does not work well. Moreover, the use of PEG to deliver mRNA is an inexpensive alternative to electroporation in that it does not require an electroporator.

Keywords

Polyethylene Glycol Plant Protoplast Equal Aliquot Calcium Pantothenate Affect Expression Level 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Abbreviations

PEG

Polyethylene glycol

DTT

dithiothreitol

MES

2(N-morpholino)ethanesulfonic acid

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Copyright information

© Springer-Verlag 1993

Authors and Affiliations

  • Daniel R. Gallie
    • 1
  1. 1.Department of BiochemistryUniversity of CaliforniaRiversideUSA

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