Plant Cell Reports

, Volume 11, Issue 5–6, pp 238–242

Agrobacterium-mediated transformation of Citrus stem segments and regeneration of transgenic plants

  • G. A. Moore
  • C. C. Jacono
  • J. L. Neidigh
  • S. D. Lawrence
  • K. Cline
Article

Summary

A method for Agrobacterium-mediated transformation of Citrus and organogenic regeneration of transgenic plants is reported. Internodal stem segments were co-cultured with Agrobacterium harboring binary vectors that contained the genes for the scorable marker ß-glucuronidase (GUS) and the selectable marker NPT-II. A low but significant percentage (≤ 5%) of the shoots regenerated in the presence of 100 μg/ml kanamycin were GUS+. Polymerase chain reaction (PCR) analysis confirmed that GUS+ shoots contained T-DNA. Two plants established in soil were shown to be transgenic by Southern analysis.

Key words

Carrizo citrange Key lime (C. aurantifoliaGenetic transformation 

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Copyright information

© Springer-Verlag 1992

Authors and Affiliations

  • G. A. Moore
    • 1
  • C. C. Jacono
    • 1
  • J. L. Neidigh
    • 1
  • S. D. Lawrence
    • 1
  • K. Cline
    • 1
  1. 1.Fruit Crops DepartmentInstitute of Food and Agricultural Sciences, University of FloridaGainesvilleUSA

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