Cell and Tissue Research

, Volume 205, Issue 2, pp 187–198 | Cite as

Pancreatic polypeptide (PP)- and glucagon cells in the pancreatic islet of Xiphophorus helleri h. (Teleostei)

Correlative Immunohistochemistry and Electron Microscopy
  • Catherine Klein
  • Susan Van Noorden
Article
Accepted:

Summary

Correlative immunohistochemical and electron microscopical studies on the pancreatic islet of the teleost fish Xiphophorus helleri using antibodies to pancreatic polypeptide (PP) and glucagon show that separate cell types are responsible for the production of these peptides. The PP-cells correspond to the previously described “A2-cells with round granules”, while the “A2-cells with crystalline granules” are the true glucagon cells. An earlier suggestion that there are two types of glucagon cells in teleost islets is therefore withdrawn.

Key words

Pancreatic polypeptide (PP) Glucagon Pancreatic islet Xiphophorus helleri Immunohistochemistry Electron microscopy 

Résumé

L'étude immunohistochimique de l'îlot pancréatique du poisson téléostéen Xiphophorus helleri, à l'aide de sérums anti-polypeptide pancréatique (PP) et anti-glucagon a permis de montrer que deux populations cellulaires distinctes sont responsables de la sécrétion de ces hormones. L'observation comparée de coupes sériées, ultrafines et semifines, traitées par la technique immunohistochimique, a démontré que les cellules à PP correspondent aux cellules qui avaient été précédemment définies, dans cette espèce, comme “cellules A2 à grains ronds” et que les cellules sécrétrices de glucagon sont les “cellules A2 à grains cristallins”. L'hypothèse de l'existence de deux catégories de cellules à glucagon chez les téléostéens est abandonnée.

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Copyright information

© Springer-Verlag 1980

Authors and Affiliations

  • Catherine Klein
    • 1
    • 2
    • 3
  • Susan Van Noorden
    • 1
    • 2
    • 3
  1. 1.Laboratoire de Zoologie et d'Embryologie expérimentaleUniversité Louis Pasteur de StrasbourgFrance
  2. 2.Laboratoire de Physiologie Comparée des Régulations, CNRSStrasbourgFrance
  3. 3.Department of HistochemistryRoyal Postgraduate Medical SchoolLondonEngland

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