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Plant Cell Reports

, Volume 10, Issue 10, pp 505–511 | Cite as

Identification of broccoli and cauliflower cultivars with RAPD markers

  • Jinguo Hu
  • Carlos F. Quiros
Article

Summary

RAPD (Random Amplified Polymorphic DNA) markers generated by 4 arbitrary 10-mer primers, discriminated 14 broccoli and 12 cauliflower cultivars (Brassica oleracea L.) by banding profiles. The size of the amplified DNA fragments ranged from 300 to 2600 base pairs. Twenty-eight percent of the markers were fixed in both broccoli and cauliflower, whereas 12.5% were specific to either crop. The rest were polymorphic in either or both crops. The markers generated by two and three primers were sufficient to distinguish each of the broccoli and cauliflower cultivars, respectively. The average difference in markers was 14.5 between broccoli and cauliflower markers, 5.8 between two broccoli cultivars and 7.9 between two cauliflower cultivars. Larger differences for each crop were found between cultivars from different seed companies than within the same company. RAPD markers provide a quick and reliable alternative to identify broccoli and cauliflower cultivars.

Key Words

Brassica oleracea genetic markers varietal identification DNA polymorphism 

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Copyright information

© Springer-Verlag 1991

Authors and Affiliations

  • Jinguo Hu
    • 1
  • Carlos F. Quiros
    • 1
  1. 1.Department of Vegetable CropsUniversity of CaliforniaDavisUSA

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