Molecular and Cellular Biochemistry

, Volume 47, Issue 2, pp 115–124 | Cite as

The fractionation and characterization of bovine tear proteins, especially lactoferrin

  • M. R. C. Banyard
  • H. A. McKenzie
Article

Summary

Gentle procedures are described for the fractionation of bovine tear fluid by a combination of centrifugation, salt precipitation, gel filtration and ion exchange chromatography. Fractions are examined by gel electrophoretic and immunological methods. Reference patterns are compiled and compared with bovine milk and serum patterns. Properties of some of the components are determined. Lactoferrin is isolated in two separate, but closely related, fractions. A nacidic protein with a molecular weight of 23 000 daltons, is also isolated. Distinct heterogeneity is observed between individual animals, suggestive of a genetic polymorphism. A method is presented for the determination of the antibacterial activity of tear fluid and its fractions.

Keywords

Molecular Weight Antibacterial Activity Genetic Polymorphism Exchange Chromatography Individual Animal 
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Copyright information

© Martinus Nijhoff/Dr W. Junk Publishers 1982

Authors and Affiliations

  • M. R. C. Banyard
    • 1
  • H. A. McKenzie
    • 1
  1. 1.Dept. of Physical Biochemistry, The John Curtin School of Medical Research, Institute of Advanced Studiesthe Australian National UniversityCanberra CityAustralia

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