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Molecular and Cellular Biochemistry

, Volume 100, Issue 2, pp 97–128 | Cite as

Enzyme stabilization: state of the art

  • Liliana Gianfreda
  • Maria Rosaria Scarfi
Invited Review

Abstract

‘Enzyme stabilization’ is one of the most important fields in basic and applied enzymology. In basic enzymology, it is of particular relevance to understand enzyme stabilization principles first elucidating how and why the enzymes lose their biological activity and then deriving structure-stability relationships existing in enzymatic molecules. In applied enzymology, the most significant goal is to achieve useful compounds by biocatalysis. Enzymes are good catalysts in terms of high catalytic and specific activity with ability to function under mild conditions. However, they are not always ideal catalysts for practical applications because they are generally unstable and they inactivate rapidly through several mechanisms. In order to enhance enzyme stability, many strategies have been pursued in recent years. The present article is an attempt to provide detailed information about these strategies.

Key words

immobilized stabilized enzymes soluble stabilized enzymes stabilization in organic solvents naturally and engineered stabilized enzymes 

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Copyright information

© Kluwer Academic Publishers 1991

Authors and Affiliations

  • Liliana Gianfreda
    • 1
  • Maria Rosaria Scarfi
    • 2
  1. 1.Dipartimento di Scienze Chimico-AgrariePortici, NapoliItaly
  2. 2.Istituto di Ricerca per l'Elettro-magnetismo e i Componenti ElettroniciIRECE-CNRNapoliItaly

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