Plant Cell Reports

, Volume 13, Issue 7, pp 411–416 | Cite as

Intertribal somatic hybrids between Brassica napus and Barbarea vulgaris — production of in vitro plantlets

  • Jan Fahleson
  • Ingrid Eriksson
  • Kristina Glimelius
Article

Abstract

Intertribal somatic hybrids were produced between Brassica napus and Barbarea vulgaris. The two species belong to the tribes Arabidae and Brassiceae, respectively, B. vulgaris is known to be cold tolerant and of interest to use as a gene donor to rapeseed. Of the plants produced in five fusion experiments six plants were verified to be hybrids by RFLP analysis utilizing one B. vulgaris-specific repetitive DNA sequence and two nuclear probes (rDNA and cruciferin) as markers. When analysing nuclear DNA content in four of these six hybrids, all had a higher DNA content than B. napus. However, mature plants could not be established outside in vitro conditions, indicating problems with compatibility between the species.

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Copyright information

© Springer-Verlag 1994

Authors and Affiliations

  • Jan Fahleson
    • 1
  • Ingrid Eriksson
    • 1
  • Kristina Glimelius
    • 1
  1. 1.Department of Plant BreedingSwedish University of Agricultural SciencesUppsalaSweden

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