Experimental Brain Research

, Volume 13, Issue 2, pp 222–238 | Cite as

Lamellar organization of hippocampal excitatory pathways

  • P. Andersen
  • T. V. P. Bliss
  • K. K. Skrede
Article

Summary

  1. 1.

    Entorhinal activation of the hippocampal cortex involves the sequential activation of a four-membered pathway: the perforant path from the entorhinal area — the mossy fibres from the dentate granule cells — the Schaffer collaterals of the CA3 pyramidal cells and finally, the CA1 pyramidal cell axons in the alveus.

     
  2. 2.

    The spatial orientation of these four fibre bundles has been studied by recording the extracellular field potentials (population spike), signalling the discharge of neurones in response to orthodromic or antidromic impulses. The height of the population spike was taken as an indicator of the number of cells discharged (see the previous paper).

     
  3. 3.

    The perforant path fibres from neighbouring parts of the entorhinal area run in a parallel fashion in a direction nearly transversely to the longitudinal axis of the hippocampus. In the dorsal part of the hippocampus, this direction was nearly sagittal, confirming Lømo (1971 a). The mossy fibres as well as the Schaffer collaterals and the alvear fibres were all found to run in the same direction. Thus, a point source of entorhinal activity projects its impulses through the four-membered pathway along a slice, or lamella, of hippocampal tissue oriented normally to the alvear surface and nearly sagittally in the dorsal part of the hippocampal formation. Also with more temporal locations of the stimulating and recording electrodes, the lamellar organization was maintained, but with a different orientation, matching the curving of the hippocampus so that the angle between the plane of the lamella and the longitudinal axis remained the same.

     
  4. 4.

    By injection of a quick-setting solution of vinyl acetate, the direction of the arteries and veins in the hippocampal formation was displayed. The branches from the artery running in the hippocampal fissure are nearly straight and are oriented in a direction similar to that of the lamellae.

     
  5. 5.

    The hippocampal cortex seems to be organized in parallel lamellae, both with regard to the neuronal and the vascular system. By means of this lamellar organization, small strips of the hippocampal cortex may operate as independent functional units, although excitatory and inhibitory transverse connections may modify the behaviour of the neighbouring lamellae.

     

Key words

Hippocampal formation Excitatory pathways Lamellar organization Rabbit 

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References

  1. Andersen, P., Bliss, T.V.P., Lømo, T., Olsen, L.I., Skrede, K.K.: Lamellar organization of hippocampal excitatory pathways. Acta physiol. scand. 76, 4A-5A (1969).Google Scholar
  2. —, Skrede, K.K.: Unit analysis of hippocampal population spikes. Exp. Brain Res. 13, 208–221 (1971).Google Scholar
  3. —, Blackstad, T.W., Lømo, T.: Location and identification of excitatory synapses on hippocampal pyramidal cells. Exp. Brain Res. 1, 236–248 (1966).Google Scholar
  4. —, Eccles, J.C., Løyning, Y.: Pathway of postsynaptic inhibition in the hippocampus. J. Neurophysiol. 27, 608–619 (1964).Google Scholar
  5. —, Lømo, T.: Mode of activation of hippocampal pyramidal cells by excitatory synapses on dendrites. Exp. Brain Res. 2, 247–260 (1966).Google Scholar
  6. —, Løyning, Y.: Interaction of various afferents on CA1 neurons and dentate granule cells. Coll. Int. C.N.R.S. No. 107, 23–45 (1962).Google Scholar
  7. —, Holmqvist, B., Voorhoeve, P.E.: Excitatory synapses on hippocampal apical dendrites activated by entorhinal stimulation. Acta physiol. scand. 66, 461–472 (1966).Google Scholar
  8. Blackstad, T.W., Brink, K., Hem, J., Jeune, B.: Distribution of hippocampal mossy fibers in the rat. An experimental study with silver impregnation methods. J. comp. Neurol. 138, 433–450 (1970).Google Scholar
  9. Fujita, Y., Sakata, H.: Electrophysiological properties of CA1 and CA2 apical dendrites of rabbit hippocampus. J. Neurophysiol. 25, 209–222 (1962).Google Scholar
  10. Hubel, D.H.: Tungsten mioroelectrode for recording from single units. Science 125, 549–550 (1957).Google Scholar
  11. Lorente de Nó, R.: Studies on the structure of the cerebral cortex II. Continuation of the study of the Ammonic system. J. Psychol. Neurol. (Lpz.) 46, 113–177 (1934).Google Scholar
  12. Lømo, T.: Patterns of activation in a monosynaptic cortical pathway: the perforant path input to the dentate area of the hippocampal formation. Exp. Brain Res. 12, 18–45 (1971 a).Google Scholar
  13. —: Potentiation of monosynaptic EPSPs in the perforant path — dentate granule cell synapse. Exp. Brain Res. 12, 46–63 (1971 b).Google Scholar
  14. Nilges, R.G.: The arteries of the mammalian cornuammonis. J.comp.Neurol. 80, 177–190 (1944).Google Scholar
  15. Ramon y Cajal, S.: Über die feinere Struktur des Ammonshornes. Z. wiss. Zool. 56, 615–663 (1893).Google Scholar
  16. —: Histologie du système nerveux de l'Homme et des Vertébrés. Paris, A. Maloine, 2, 993 pp. (1911).Google Scholar
  17. Spencer, W.A., Kandel, E.R.: Hippocampal neuron responses to selective activation of recurrent collaterals of hippocampofugal axons. Exp. Neurol. 4, 149–161 (1961).Google Scholar
  18. Woelcke, M.: Eine neue Methode der Markscheidenfärbung. J. Psychol. Neurol. (Lpz.), 51, 199–202 (1942).Google Scholar

Copyright information

© Springer-Verlag 1971

Authors and Affiliations

  • P. Andersen
    • 1
  • T. V. P. Bliss
    • 1
  • K. K. Skrede
    • 1
  1. 1.Institute of NeurophysiologyUniversity of OsloNorway
  2. 2.National Institute for Medical ResearchLondon

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