Cell and Tissue Research

, Volume 197, Issue 2, pp 325–336 | Cite as

Maturation of the hypothalamo-neurohypophysial system

I. localization of nenrophysin, oxytocin and vasopressin in the hypothalamus and neural lobe of the developing rat brain
  • V. J. Choy
  • W. B. Watkins
Article

Summary

Sections of the hypothalamus, median eminence and pituitary from fetal and neonatal rats were examined with the immunoperoxidase staining technique and light microscopy. Purified antisera raised against vasopressin and oxytocin, and antisera cross-reactive with rat neurophysin were used to localize these antigens in the hypothalamo-neurohypophysial system (HNS). Neurophysin was detected throughout the HNS of the 18-day fetal rat. Vasopressin was present in the hypothalamus and pituitary of the 19-day fetus, and in the median eminence of the 4-day neonate. Oxytocin was not detected in the pituitary until 1–2 days after birth, in the hypothalamus after 4 days, and in the median eminence after 8 days. During the first days after birth the supraoptic nucleus was more mature than the paraventricular nucleus. The HNS did not approach maturity until at least 7 days after birth. The relative maturity of the supraoptic nucleus compared with the paraventricular nucleus, and the detection of vasopressin before oxytocin are evidence for the one-neuron-one-hormone theory. The data do not exclude the possibility that the fetal hypothalamo-neurohypophysial system, and perhaps the fetal hormone, vasotocin, affect the initiation and course of parturition.

Key words

Rat hypothalamo-neurohypophysial system Fetal development Oxytocin Vasopressin Neurophysin 

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References

  1. Bowie, H.P., Herbert, D.C.: Immunocytochemical evidence for the presence of arginine vasotocin in the rat pineal gland. Nature 261, 66 (1976)Google Scholar
  2. Campbell, H.J.: The development of the primary portal plexus in the median eminence of the rat. J. Anat. (Lond.) 100, 381–387 (1966)Google Scholar
  3. Cannatta, M.A., Tramezzani, J.H.: Ultrastructural maturation of the neurohypophysis of the rat. Acta Anat. 97, 213–223 (1977)Google Scholar
  4. Chard, T., Silman, R.E., Rees, L.H.: the fetal hypothalamus and pituitary in the initiation of labour. In: The Fetus and Birth. Ciba Foundation Symposium 47, pp. 359–370. Amsterdam: Elsevier North-Holland 1977Google Scholar
  5. Choy, V.J., Watkins, W.B.: Immunocytochemical study of the hypothalamo-neurohypophysial system. II. Distribution of neurophysin, vasopressin and oxytocin in the normal and osmotically stimulated rat. Cell Tissue Res. 189, 467–490 (1977)Google Scholar
  6. Dicker, S.E.: Release of vasopressin and oxytocin from isolated pituitary glands of adult and newborn rats. J. Physiol. (Lond.) 185, 429–444 (1966)Google Scholar
  7. Donev, S.: Occurrence of neurosecretory substance during the embryonic development of the guinea pig. Z. Zellforsch. 104, 517–529 (1970)Google Scholar
  8. Fink, G., Smith, G.C.: Ultrastructural features of the developing hypothalamo-hypophysial axis in the rat. A correlative study. Z. Zellforsch. 119, 208–226 (1971)Google Scholar
  9. Forsling, M.L.: In: Endocrine Factors and Labour, Memoirs Soc. Endocrinol. 20, p. 174 (A. Klopper and J. Gardner, eds.) London: Cambridge Univ. Press 1973Google Scholar
  10. Graham, R.C., Karnovsky, M.J.: The early stages of absorption of injected horseradish peroxidase in the proximal tubules of mouse kidney; ultrastructural cytochemistry by a new technique. J. Histochem. Cytochem. 14, 291–302 (1966)Google Scholar
  11. Heller, H., Lederis, K.: Maturation of the hypothalamo-neurohypophysial system. J. Physiol. (Lond.) 147, 299–314 (1959)Google Scholar
  12. LeClerc, R., Pelletier, G.: Ontogeny of neurophysin in the rat pituitary gland. An electron microscope immunohistochemical study. Brain Res. 129, 275–281 (1977)Google Scholar
  13. Liggins, G.C.: Hormonal interactions in the mechanism of parturition. In: Endocrine Factors in Labour, Memoirs Soc. Endocrinol. 20, pp. 119–139 (A. Klopper and J. Gardner, eds.) London: Cambridge Univ. Press 1973Google Scholar
  14. Mason, T.E., Phifer, R.F., Spicer, S.S., Swallow, R.A., Dreskin, R.B.: An immunoglobulin-enzyme bridge method for localising tissue antigens. J. Histochem. Cytochem. 17, 563–569 (1969)Google Scholar
  15. Perks, A.M., Vizsolyi, E.: Studies of the neurohypophysis in foetal mammals. In: Foetal and Neonatal Physiology, Proc. Barcroft Symposium, pp. 430–438. (K.S. Comline, K.W. Cross, G.S. Dawes and P.W. Nathanielsz, eds.) London: Cambridge Univ. Press 1973Google Scholar
  16. Rinne, U.K., Kivalo, E.: Maturation of hypothalamic neurosecretion in rat, with special reference to the neurosecretory material passing into the hypophysial portal system. Acta Neurovegetative 27, 166–183 (1964)Google Scholar
  17. Silverman, A.J.: The hypothalamic magnocellular neurosecretory system of the guinea pig. II. Immunohistochemical localisation of neurophysin and vasopressin in the fetus. Am. J. Anat. 144, 445–460 (1975)Google Scholar
  18. Smith, C.S., Simpson, R.W.: Monoamine fluorescence in the median eminence of foetal, neonatal and adult rats. Z. Zellforsch. 104, 541–556 (1970)Google Scholar
  19. Sternberger, L.A., Hardy, P.H., Cuculis, J.J., Meyer, H.G.: The unlabeled antibody enzyme method of immunohistochemistry. Preparation and properties of soluble antigen-antibody complex (horseradish peroxidase-antihorseradish peroxidase) and its use in identification of spirochetes. J. Histochem. Cytochem. 18, 315–333 (1970)Google Scholar
  20. Swaab, D.F., Boer, K., Honnebier, W.J.: The influence of the fetal hypothalamus and pituitary on the onset and course of parturition. In: The Fetus and Birth. Ciba Foundation Symposium 47, pp. 379–393. Amsterdam: Elsevier North-Holland 1977Google Scholar
  21. Swaab, D.F., Pool, C.W.: Specificity of oxytocin and vasopressin immunofluorescence. J. Endocr. 66, 263–272 (1975)Google Scholar
  22. Watkins, W.B.: Immunohistochemical demonstration of neurophysin in the hypothalamoneurohypophysial system. Int. Rev. Cyto. 41, 241–284 (1975)Google Scholar

Copyright information

© Springer-Verlag 1979

Authors and Affiliations

  • V. J. Choy
    • 1
  • W. B. Watkins
    • 1
  1. 1.Postgraduate School of Obstetrics and GynaecologyUniversity of AucklandAucklandNew Zealand

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