Plant Cell Reports

, Volume 14, Issue 4, pp 245–248 | Cite as

Totipotency of coleoptile tissue in indica rice (Oryza sativa L. cv. ch 1039)

  • G. S. Oinam
  • S. L. Kothari


Embryogenic and non-embryogenic calluses were induced from 3,4,5 and 7d old coleoptile segments of indica rice (Oryza sativa L. cv. CH 1039). Compact, globular, yellow and creamy embryogenic and white friable non-embryogenic callus arose from the cut end and entire length of the coleoptile segments. Murashige and Skoog's (MS) medium supplemented with 2.5mg/1 2,4-D was used as callus induction medium. Plant regeneration from coleoptile segments occurred with the transfer of embryogenic callus to MS basal medium supplemented with 2.0mg/1 BAP and 0.5mg/1 NAA in combination. Average number of regenerated plants from one coleoptile ranged from9.1 to 14.0.Four day old coleoptiles showed the highest frequency of plant regeneration.


Basal Medium Plant Regeneration Entire Length Callus Induction Induction Medium 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.



2,4-dichlorophenoxyacetic acid




Murashige and Skoog (1962)


1-naphthalene acetic acid


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Copyright information

© Springer-Verlag 1995

Authors and Affiliations

  • G. S. Oinam
    • 1
  • S. L. Kothari
    • 1
  1. 1.Department of BotanyUniversity of RajasthanJaipurIndia

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