Advertisement

Plant Cell Reports

, Volume 14, Issue 4, pp 245–248 | Cite as

Totipotency of coleoptile tissue in indica rice (Oryza sativa L. cv. ch 1039)

  • G. S. Oinam
  • S. L. Kothari
Article

Abstract

Embryogenic and non-embryogenic calluses were induced from 3,4,5 and 7d old coleoptile segments of indica rice (Oryza sativa L. cv. CH 1039). Compact, globular, yellow and creamy embryogenic and white friable non-embryogenic callus arose from the cut end and entire length of the coleoptile segments. Murashige and Skoog's (MS) medium supplemented with 2.5mg/1 2,4-D was used as callus induction medium. Plant regeneration from coleoptile segments occurred with the transfer of embryogenic callus to MS basal medium supplemented with 2.0mg/1 BAP and 0.5mg/1 NAA in combination. Average number of regenerated plants from one coleoptile ranged from9.1 to 14.0.Four day old coleoptiles showed the highest frequency of plant regeneration.

Keywords

Basal Medium Plant Regeneration Entire Length Callus Induction Induction Medium 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Abbreviations

2,4-D

2,4-dichlorophenoxyacetic acid

BAP

6-benzylaminopurine

MS

Murashige and Skoog (1962)

NAA

1-naphthalene acetic acid

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. Abdullah R, Cocking EC, Thompson JA (1986) Bio/Technol 4: 1087–1090Google Scholar
  2. Abe T, Futsuhara Y (1986) Theor Appl Genet 72: 3–10Google Scholar
  3. Bhaskaran S, Smith RH (1990) Crop Sci 30: 1328–1337Google Scholar
  4. Bhattacharya P, Sen SK (1980) Theor Appl Genet 58: 87–90Google Scholar
  5. Christou P, Ford TL, Kofron M (1992) Trends Biotech 10: 239–246Google Scholar
  6. Datta K, Potrykus I, Datta SK (1992) Plant Cell Rep 11: 229–233Google Scholar
  7. Ghosh Biswas GC, Zapata FZ (1993) J Plant Physiol 141: 470–475Google Scholar
  8. Kawata S, Ishihara A (1968) Proc Japan Acad 44: 549–552Google Scholar
  9. Koetje DS, Grimes HD, Wang YC, Hodges TK (1989) J Plant Physiol 135: 184–190Google Scholar
  10. Lee L, Schroll RE, Grimes HD, Hodges TK, (1989) Planta 178: 325–333Google Scholar
  11. Murashige T, Skoog F (1962) Physiol Plant 15: 473–497Google Scholar
  12. Nakano H, Tashiro T, Maeda E (1975) Z Pflanzenphysiol 76: 444–449Google Scholar
  13. O'Hara JF, Street HE (1978) Ann Bot 42: 1029–1038Google Scholar
  14. Oinam GS, Kothari SL (1993) Intl Rice Res Notes 18: 9–10Google Scholar
  15. Shu LH, Wei JY (1980) Acta Wuhan University Jour 1: 94–100Google Scholar
  16. Sticklen MB (1991) J Plant Physiol 138: 577–580Google Scholar
  17. Torrizo LB, Zapata FZ (1992) J Exp Bot 43: 1633–1637Google Scholar
  18. Tsai IS, Kei KL, Chou KT, Yang CS, Yang LF (1978) In: Proceedings of Symposium on Plant Tissue Culture, Science Press, Peking: pp 517–519Google Scholar
  19. Wu L, Li HW (1971) Cytologia 36: 411–416Google Scholar
  20. Wu KQ, Chen Y (1989) Acta Botanic Austro Sinica 5: 141–147Google Scholar
  21. Yin Y, Li S, Chen Y, Guo H, Tian W, Chen Y, Li L (1993) Plant Cell Tissue Organ Culture 32: 61–68Google Scholar

Copyright information

© Springer-Verlag 1995

Authors and Affiliations

  • G. S. Oinam
    • 1
  • S. L. Kothari
    • 1
  1. 1.Department of BotanyUniversity of RajasthanJaipurIndia

Personalised recommendations