Cell and Tissue Research

, Volume 197, Issue 3, pp 515–530 | Cite as

The juxtaligamental cells of Ophiocomina nigra (Abildgaard) (Echinodermata: Ophiuroidea) and their possible role in mechano-effector function of collagenous tissue

  • I. C. Wilkie


The intervertebral ligament of the brittlestar Ophiocomina nigra contains numerous cellular processes which belong to perikarya located on the outer surfaces of the ligament. These are described as the juxtaligamental cells and have been studied by light and electron microscopy. The cells are mainly concentrated in four pairs of ganglion-like nodes associated with the intervertebral ligament and in similar nodes adjacent to every other major connective tissue component of the arm. Although their histochemistry and ultrastructure indicate a neurosecretory function, they are anomalous in containing unusually large electron-dense granules probably associated with calcium. The ganglion-like nodes are innervated by hyponeural nerves, though synaptic contacts with the juxtaligamental cells have yet to be demonstrated.

The function of the cells is discussed and it is suggested that they may be involved in the rapid loss of tensile strength which the intervertebral ligament sustains during arm autotomy. They may achieve this by controlling the availability of Ca2+ ions to the extracellular compartment of the ligament.

A version of this paper was read at the U.K.-Eire Echinoderms Colloquium, Bedford College, London, in July 1978

Key words

Echinodermata juxtaligamental cells Collagenous tissue Ophiocomina nigra Neurosecretory cells 


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  1. Bullock, T.H., Horridge, G.A.: Structure and function in the nervous systems of invertebrates. 2 vols. New York: W.H. Freeman 1965Google Scholar
  2. Cobb, J.L.S., Sneddon, E.: An ultra-structural study of the gills of Echinus esculentus. Cell Tissue Res. 182, 265–274 (1977)Google Scholar
  3. Drury, R.A.B., Wallington, E.A.: Carleton's histological technique. 4th edn. Oxford: University Press 1967Google Scholar
  4. Fawcett, D.W.: The cell. Philadelphia: Saunders 1966Google Scholar
  5. Finlayson, L.H., Osborne, M.P.: Secretory activity of neurons and related electrical activity. Adv. Comp. Physiol. Biochem. 6, 165–258 (1975)Google Scholar
  6. Fontaine, A.R.: Neurosecretion in the ophiuroid Ophiopholis aculeata. Science (N.Y.) 138, 908–909 (1962)Google Scholar
  7. Hagadorn, I.R.: Neurosecretory mechanisms. In: Invertebrate nervous systems (C.A.G. Wiersma, ed.). Chicago: University Press 1967Google Scholar
  8. Hamann, O.: Anatomie der Ophiuren und Crinoiden. Jena Z. Naturw. 23, 233–388 (1889)Google Scholar
  9. Heath, I.D.: Observations on a highly specific method for the histochemical detection of sulphated mucopolysaccharides, and its possible mechanisms. Quart. J. Micr. Sci. 103, 457–475 (1962)Google Scholar
  10. Hehn, G. von: Über den Feinbau des hyponeuralen Nervensystems des Seesternes (Asterias rubens L.). Z. Zellforsch. 105, 137–154 (1970)Google Scholar
  11. Holland, N.D.: The fine structure of the axial organ of the feather star Nemaster rubiginosa (Echinodermata: Crinoidea). Tissue Cell 2, 625–636 (1970)Google Scholar
  12. Humason, G.L.: Animal tissue techniques. San Francisco: W.H. Freeman 1967Google Scholar
  13. Hunt, S.: Polysaccharide-protein complexes in invertebrates. London: Academic Press 1970Google Scholar
  14. Leclerc, M., Delavault, R.: Présence de fibres nerveuses dans la paroi du sinus axial et dans la paroi coelomique chez Asterina gibbosa Pennant (Echinoderme, Astéride). C.R. Acad. Sci. [D] (Paris) 272, 3311–3313 (1971)Google Scholar
  15. Lente, T.L.: Cell fine structure. Philadelphia: Saunders 1971Google Scholar
  16. Lillie, R.D.: Histopathologic technic and practical histochemistry. 3rd. edn. New York: McGraw-Hill 1965Google Scholar
  17. Luft, J.H.: Ruthenium red and violet I. Chemistry, purification, methods of use for electron microscopy and mechanism of action. Anat. Rec. 171, 347–368 (1971)Google Scholar
  18. Meek, G.A.: Apparent intracellular collagen synthesis. In: Cell structure and its interpretation (S.M. McGee-Russel and K.F.A. Ross, eds.). London: Edward Arnold 1968Google Scholar
  19. Nicaise, G.: The gliointerstitial system of molluscs. Int. Rev. Cytol. 34, 251–332 (1973)Google Scholar
  20. Pearse, A.G.E.: Histochemistry. Theoretical and applied. Vol. I. London: Churchill 1968Google Scholar
  21. Pentreath, V.W., Cottrell, G.A.: ‘Giant’ neurons and neurosecretion in the hyponeural tissue of Ophiothrix fragilis Abild. J. Exp. Mar. Biol. Ecol. 6, 249–264 (1971)Google Scholar
  22. Singer, I.I.: An electron microscopic and autoradiographic study of mesogleal organization and collagen synthesis in the sea anemone Aiptasia diaphana. Cell Tissue Res. 149, 537–554 (1974)Google Scholar
  23. Takahashi, K.: The catch apparatus of the sea-urchin spine I. Gross histology. J. Facul. Sci., Univ. Tokyo IV 11, 109–120 (1967a)Google Scholar
  24. Takahashi, K.: The catch apparatus of the sea-urchin spine II. Responses to stimuli. J. Facul. Sci., Univ. Tokyo IV 11, 121–130 (1967b)Google Scholar
  25. Tombes, A.S.: An introduction to invertebrate endocrinology. New York: Academic Press 1970Google Scholar
  26. Wilkie, I.C.: A study of the process of ophiuroid arm autotomy. Ph. D. thesis, University of Glasgow 1976Google Scholar
  27. Wilkie, I.C.: Arm autotomy in brittlestars (Echinodermata: Ophiuroidea). J. Zool. (Lond.) 186, 311–330 (1978a)Google Scholar
  28. Wilkie, I.C.: Nervously mediated change in the mechanical properties of a brittlestar ligament. Mar. Behav. Physiol. 5, 289–306 (1978b)Google Scholar
  29. Yahata, T., Takahashi, H.: Demonstration of possible neurosecretory cells in the nervous system of neptune whelk, Neptunea arthritica. Bull. Fac. Fish. Hokkaido Univ. 23, 135–143 (1972)Google Scholar

Copyright information

© Springer-Verlag 1979

Authors and Affiliations

  • I. C. Wilkie
    • 1
  1. 1.Bioengineering UnitUniversity of StrathclydeGlasgowScotland

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