Plant Cell Reports

, Volume 11, Issue 7, pp 323–328 | Cite as

Development of the particle inflow gun for DNA delivery to plant cells

  • John J. Finer
  • Philippe Vain
  • Mark W. Jones
  • Michael D. McMullen
Article

Summary

A simple and inexpensive particle bombardment device was constructed for delivery of DNA to plant cells. The Particle Inflow Gun (PIG) is based on acceleration of DNA-coated tungsten particles using pressurized helium in combination with a partial vacuum. The particles are accelerated directly in a helium stream rather than being supported by a macrocarrier. Bombardment parameters were partially optimized using transient expression assays of a ß-glucuronidase gene in maize embryogenic suspension culture and cowpea leaf tissues. High levels of transient expression of the ß-glucuronidase gene were obtained following bombardment of embryogenic suspension cultures of corn and soybean, and leaf tissue of cowpea. Stable transformation of embryogenic tissue of soybean has also been obtained using this bombardment apparatus.

Abbreviations

2,4-D

2,4-dichlorophenoxyacetic acid

PCV

packed cell volume

GUS

ß-glucuronidase

NOS

nopaline synthase

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. Finer JJ, MD McMullen (1990) Plant Cell Rep 8:586–589.Google Scholar
  2. Finer JJ, MD McMullen (1991) In Vitro Cell Dev Biol 27P:175–182.Google Scholar
  3. Gamborg OL, RA Miller, K Ojima (1968) Exp Cell Res 50:151–158.PubMedGoogle Scholar
  4. Gordon-Kamm WJ, TM Spencer, ML Mangano, TR Adams, RJ Daines, WG Start, JV O'Brien, SA Chambers, WR Adams, NW Willetts, TB Rice, CJ Mackey, RW Krueger, AP Kausch, PG Lemaux (1990) The Plant Cell 2:603–618.Google Scholar
  5. Gritz L, J Davies (1983) Gene 26:179–188.Google Scholar
  6. Jefferson RA, TA Kavanagh, MW Bevan (1987) The EMBO Jour 6:3901–3907.Google Scholar
  7. Jefferson RA (1987) Plant Mol Biol Rep 5:387–405.Google Scholar
  8. Johnston SA, M Riedy, MJ DeVit, JC Sanford, S McElligott, RS Williams (1991) In Vitro Cell Dev Biol 27:11–14.Google Scholar
  9. Klein TM, ED Wolf, R Wu, JC Sanford (1987) Nature 327:70–73.Google Scholar
  10. Klein TM, M Fromm, A Weissinger, D Tomes, S Schaff, M Sletten, JC Sanford (1988) Proc Natl Acad Sci 85:4305–4309.Google Scholar
  11. McCabe DE, WF Swain, BJ Martineil, P Christou (1988) Bio/Technology 6:923–926.Google Scholar
  12. Morikawa H, A Iida, Y Yamada (1989) Appl Microbiol Biotechnol 31:320–322.Google Scholar
  13. Murashige T, F Skoog (1962) Physiol Plant 15:474–497.Google Scholar
  14. Oard JH, DF Paige, JA Simmonds, TM Gradziel (1990) Plant Physiol 92:334–339.Google Scholar
  15. Takeuchi Y, M Dotson, NT Keen (1992) Plant Molec Biol (In press).Google Scholar
  16. Sanford JC, TM Klein, ED Wolf, N Allen (1987) Particulate Sci Technol 5:27–37.Google Scholar
  17. Sautter C, H Waldner, G Neuhaus-Url, A Galli, G Niehaus, I Potrykus (1991) BioTechnology 9:1080–1085.Google Scholar
  18. Vain P, H Yean, P Flament (1989) Plant Cell Tiss Org Cult 18:143–151.Google Scholar
  19. Vasil V, M Clancy, RJ Ferl, IK Vasil, LC Hannah (1989) Plant Physiol 91:1575–1579.Google Scholar

Copyright information

© Springer-Verlag 1992

Authors and Affiliations

  • John J. Finer
    • 1
  • Philippe Vain
    • 1
  • Mark W. Jones
    • 1
  • Michael D. McMullen
    • 1
    • 2
  1. 1.Department of Agronomy, The Ohio Agricultural Research and Development CenterThe Ohio State UniversityWoosterUSA
  2. 2.Corn and Soybean Research UnitAgricultural Research ServiceUSDA

Personalised recommendations