The Journal of Membrane Biology

, Volume 145, Issue 1, pp 99–106 | Cite as

Synergistic action of vasopressin and aldosterone on basolateral Na+-K+-ATPase in the cortical collecting duct

  • N. Coutry
  • N. Farman
  • J. P. Bonvalet
  • M. Blot-Chabaud
Articles
Received:
Revised:

Abstract

The respective effects of aldosterone and arginine vasopressin (AVP) were examined on the number of active Na+-K+-ATPase and their pumping activity in nonperfused microdissected mouse cortical collecting tubules (CCD) by measuring specific 3H-ouabain binding and ouabain-sensitive 86Rb uptake. In adrenalectomized (ADX) animals, incubation of CCD with AVP (10−8m for 5 min) had no effect on the number of pumps. In contrast, in ADX animals replete with aldosterone, AVP induced a ≅40% increase in the number of pumps. This was accompanied by a ≅60–65% increase in ouabain-sensitive Rb uptake. AVP effect was dose-dependent (10−10–10−8m) and was reproduced by dDAVP, forskolin and 8-Br cAMP, indicating a V2 pathway. It was inhibited by amiloride 10−5m, and did not occur in CCD incubated in hyperosmotic solution, suggesting that the signal was transmitted via apical sodium entry and cell swelling. Finally, the AVP-dependent increase in the number of pumps was rapid (within 5 min) and transient (<25 min).

These results demonstrate that, in the CCD, aldosterone and AVP act synergistically to increase not only the apical sodium entry but also the basolateral Na+-K+-ATPase transport capacity: AVP allows a rapid recruitment and/or activation of an aldosterone-dependent pool of latent Na+-K+-ATPase.

Key words

Mineralocorticoids Mouse kidney Ouabain binding Rubidium uptake Sodium transport 
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Copyright information

© Springer-Verlag New York Inc. 1995

Authors and Affiliations

  • N. Coutry
    • 1
  • N. Farman
    • 1
  • J. P. Bonvalet
    • 1
  • M. Blot-Chabaud
    • 1
  1. 1.INSERM U426, IFR ≪Cellules Epithéliales≫, Faculté X. BichatParis CédexFrance

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