Plant Cell Reports

, Volume 14, Issue 1, pp 59–64 | Cite as

Stable expression of the GUS reporter gene in chrysanthemum depends on binary plasmid T-DNA

  • Jan de Jong
  • Marleen M. J. Mertens
  • Wim Rademaker
Article
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Abstract

Three chrysanthemum (Dendranthema grandiflora) cultivars were cocultivated with 2 Agrobacterium tumefaciens strains in combination with 4 pBIN19 derived binary plasmids, all carrying the Nosnptll selection gene and 35Sgus(intron) reporter gene. All binary plasmids transferred DNA to chrysanthemum explants but only pMOG410 gave good stable expression of GUS. This plasmid differs from the other plasmids in 2 aspects: 1) It carries a restored nptll gene and 2) the selection gene is positioned at the left border side of the reporter gene. Cocultivation with AGLO(pMOG410) yielded up to 13 GUS positive shoots per 100 explants. The presence of the gus and nptll gene in recovered shoots was confirmed by PCR and Southern blot analysis.

Key words

Agrobacterium transformation T-DNA 
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Copyright information

© Springer-Verlag 1994

Authors and Affiliations

  • Jan de Jong
    • 1
  • Marleen M. J. Mertens
    • 2
  • Wim Rademaker
    • 1
  1. 1.DLO-Centre for Plant Breeding and Reproduction Research (CPRO-DLO)AA WageningenThe Netherlands
  2. 2.Research Station for Ornamental Plant GrowingMolloBelgium

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