Experimental Brain Research

, Volume 19, Issue 3, pp 314–325 | Cite as

Estimations histophotométriques et dosages biochimiques des orthophosphorique-monoester-phosphohydrolases acides du système hypothalamo-neurohypophysaire du rat soumis à une épreuve de déshydratation

  • Arlette Burlet
Article
Received:

Histophotometric estimations and biochemical assays of acid orthophosphoric-monoester-phosphohydrolases in the hypothalamo-neurohypophyseal system of the rat during dehydration

Summary

The activities of acid orthophosphoric-monoester-phosphohydrolases in the supra-opticus nucleus and the neurohypophysis of the rat were measured at pH 4.1 and 5.3 by biochemical assay and by histospectrophotometric method after a thirsting period of 1–12 days. For the neurohypophysis the biochemical and histophotometrical results are convergent. These enzymatic activities increase during the entire experimental period but if the biochemical results are expressed for 100 μg of proteins the elevation is only significant at pH 4.1. For the supra-optic nucleus, changes in enzymatic activities are different by histophotometric and biochemical methods. It is assumed that in the rat hypothalamo-neurohypophyseal system the acid phosphatases are distributed between two separate pools: (1) one strongly bound to cytoplasmic membrane structures, particulary abundant in the perikarya of the supra-optic nucleus, and (2) another pool of “free” or easily discharged enzyme, the major component in the neurohypophysis; our biochemical assay measures only this “free pool”. The role of these pools in the synthesis and release of vasopressin is presumably different.

Key words

Hypothalamo-neurohypophyseal system Dehydration Acid phosphatases Biochemistry Histochemistry 
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Copyright information

© Springer-Verlag 1974

Authors and Affiliations

  • Arlette Burlet
    • 1
  1. 1.Laboratoire d'histologie, U.E.R. Sciences médicales ANancyFrance

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