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Plant Cell Reports

, Volume 15, Issue 11, pp 869–872 | Cite as

Phenylacetic acid induced organogenesis in cultured leaf segments of Dianthus chinensis

  • Vidya Jethwani
  • S. L. Kothari
Article

Summary

Shoot regeneration was achieved from leaf derived callus of Dianthus chinensis using Phenylacetic acid (PAA). Callus from basal leaf segments, raised on Murashige and Skoog's (MS) medium containing 2,4-dichlorophenoxyacetic acid (2,4-D) or 1-Naphthaleneacetic acid (NAA) in combination with 6-benzylamino purine (BAP), was subcultured on medium supplemented with BAP in combination with 2,4-D, NAA or PAA. Shoots were induced only when leaf derived callus was subcultured on medium containing BAP (2.0, 5.0 mg/l) in combination with PAA (0.5, 1.0 mg/l). No shoot regeneration was observed when 2,4-D, NAA or BAP were used in the medium either singly or in different combinations. These results demonstrate that PAA in combination with BAP was essential to trigger shoot regeneration from cultured leaf callus of D. chinensis.

Keywords

Purine Shoot Regeneration Basal Leaf Leaf Segment Phenylacetic Acid 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Abbreviations

BAP

6-benzylaminopurine

2,4-D

2,4-dichlorophenoxyacetic acid

DPX

dibutylphthalate xylol

MS

Murashige and Skoog (1962) basal medium

NAA

1-Naphthaleneacetic acid

PAA

Phenylacetic acid

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Copyright information

© Springer-Verlag 1996

Authors and Affiliations

  • Vidya Jethwani
    • 1
  • S. L. Kothari
    • 1
  1. 1.Department of BotanyUniversity of RajasthanJaipurIndia

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