Plant Cell Reports

, Volume 15, Issue 11, pp 869–872 | Cite as

Phenylacetic acid induced organogenesis in cultured leaf segments of Dianthus chinensis

  • Vidya Jethwani
  • S. L. Kothari


Shoot regeneration was achieved from leaf derived callus of Dianthus chinensis using Phenylacetic acid (PAA). Callus from basal leaf segments, raised on Murashige and Skoog's (MS) medium containing 2,4-dichlorophenoxyacetic acid (2,4-D) or 1-Naphthaleneacetic acid (NAA) in combination with 6-benzylamino purine (BAP), was subcultured on medium supplemented with BAP in combination with 2,4-D, NAA or PAA. Shoots were induced only when leaf derived callus was subcultured on medium containing BAP (2.0, 5.0 mg/l) in combination with PAA (0.5, 1.0 mg/l). No shoot regeneration was observed when 2,4-D, NAA or BAP were used in the medium either singly or in different combinations. These results demonstrate that PAA in combination with BAP was essential to trigger shoot regeneration from cultured leaf callus of D. chinensis.


Purine Shoot Regeneration Basal Leaf Leaf Segment Phenylacetic Acid 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.





2,4-dichlorophenoxyacetic acid


dibutylphthalate xylol


Murashige and Skoog (1962) basal medium


1-Naphthaleneacetic acid


Phenylacetic acid


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. Abe U, Uchiyama M, Sato R (1974) Agric Biol Chem 38: 897–898Google Scholar
  2. Gifford EM Jr, Corson GE Jr (1971) Bot Rev 37: 143–229Google Scholar
  3. Isogai Y, Okamoto T, Koizumi T (1967) Chem Pharm Bull (Tokyo) 15: 151–158Google Scholar
  4. Jahne A, Lazzeri A, Jager-Gussen M, Lorz H (1991) Theor Appl Genet 82: 74–80Google Scholar
  5. Jethwani V, Kothari SL (1993) Plant Tissue Cult 3: 91–96Google Scholar
  6. Jethwani V, Kothari SL (1994) Indian Hort, July–Sept, 38Google Scholar
  7. Jethwani V, Sharma VK, Kothari SL (1994) J Indian Bot Soc 73: 357–358Google Scholar
  8. Johansen DA (1940) Plant Microtechnique. McGraw Hill, New YorkGoogle Scholar
  9. Kothari SL, Chandra N (1984) J Plant Physiol 117: 105–108Google Scholar
  10. Leuba V, LeTourneau D (1990)J Plant Growth Regul 9: 71–76Google Scholar
  11. Mii M, Buiatti M, Gimelli F (1990) Carnation. In: Ammirato PV, Evans DR, Sharp WR, Bajaj YPS (eds) Handbook of plant cell culture, ornamental species, vol. 5, Macmillan, New York, pp 284–318Google Scholar
  12. Milborrow BV, Purse JG, Wightman F (1975) Ann Bot 39: 1143–1146Google Scholar
  13. Miller RM, Kaul V, Hutchinson JF, Richards D (1991) Ann Bot 67: 35–42Google Scholar
  14. Murashige T, Skoog F (1962) Physiol Plant 15: 473–497Google Scholar
  15. Nakano M, Mii M (1992) Plant Cell Rep 11: 225–228Google Scholar
  16. Nakano M, Mii M (1993) Theor Appl Genet 86: 1–5Google Scholar
  17. Roest S, Bokelman GS (1981) Scientia Hort 14: 357–366Google Scholar
  18. Sankhla D, Davis TD, Sankhla N, Upadhyaya A (1994) Plant Cell Rep 13: 514–518Google Scholar
  19. Schneider EA, Wightman F (1986) Physiol Plant 68: 396–402Google Scholar
  20. Slimmon T, Qureshi JA, Saxena PK (1991) Plant Cell Rep 10: 587–589Google Scholar
  21. Small DK, Morris DA (1990) Plant Growth Regul 19: 329–340Google Scholar
  22. Takeda Y (1974) Special Rep Shiga Pref Agric Exp Sta 11: 1–124Google Scholar
  23. Wightman F, Schneider EA, Thimann KV (1980) Physiol Plant 49: 304–314Google Scholar
  24. Van Altvorst AC, Koehorst JJ, Bruinsma T, Jansen J, Custers JBM, Jong J, Dons JJM (1992) Scientia Hort 51: 223–235Google Scholar
  25. Van Altvorst AC, Koehorst HJJ, Bruinsma T, Dons JJM (1994) Plant Cell Tissue Organ Cult 37: 87–90Google Scholar
  26. Van Altvorst AC, Tancheva S, Dons J (1995) Plant Cell Tissue Organ Cult 40: 151–157Google Scholar

Copyright information

© Springer-Verlag 1996

Authors and Affiliations

  • Vidya Jethwani
    • 1
  • S. L. Kothari
    • 1
  1. 1.Department of BotanyUniversity of RajasthanJaipurIndia

Personalised recommendations