Advertisement

Plant Cell Reports

, Volume 15, Issue 11, pp 859–864 | Cite as

Regeneration of plants from cryopreserved embryogenic cell suspension and callus cultures of cotton (Gossypium hirsutum L.)

  • Kanniah Rajasekaran
Article

Abstract

Successful regeneration of cotton (Gossypium hirsutum L.) plants from cryopreserved embryogenic callus and cell suspension cultures is described. The cryoprotectant mixture consisting of a modified Murashige and Skoog (1962) medium with sucrose (5% w/v), DMSO (5% v/v) and glycerol (5% v/v) gave the highest survival rate (70%) from cell suspension cultures cryopreserved in liquid nitrogen after slow cooling (0.5 to 1.0°C/min). A cooling rate of 0.5°C/min provided a satisfactory recovery rate (30%) from cryopreserved embryogenic callus cultures and was superior to a cooling rate of 1°C/min. Regenerated plants from cell suspension and embryogenic callus cultures cryopreserved for more than four years exhibited normal morphology, growth and boll set upon transfer to soil.

Keywords

Sucrose Glycerol DMSO Cell Suspension Cooling Rate 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Abbreviations

DMSO

dimethylsulfoxide

MS

Murashige and Skoog (1962)

MMS

modified MS

NAA

α-naphthaleneacetic acid

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. Bajaj YPS and Gill MS (1985) In vitro induction of genetic variability in cotton (Gossypium spp.). Theor Appl Genet 70: 363–368.Google Scholar
  2. Chen THH, Kartha, KK, Constabel F and Gusta LV (1984) Freezing characteristics of cultured Catharanthus roseus (L.) G. Don cells treated with dimethylsulfoxide and sorbitol in relation to cryopreservation. Plant Physiol 75: 720–725.Google Scholar
  3. Eksomtramage T, Paulet F, Guilderdoni E, Glaszmann JC and Engelmann F (1992) Development of a cryopreservation process for embryogenic calluses of a commercial hybrid of sugarcane (Saccharum sp.) and application to different varieties. Cryo-Letters 13: 239–252.Google Scholar
  4. Finkle BJ and Ulrich JM (1979) Effects of cryoprotectants in combination on the survival of frozen sugarcane cells. Plant Physiol 63: 598–604.Google Scholar
  5. Gnanapragasam S and Vasil IK (1990) Plant regeneration from a cryopreserved embryogenic cell suspension of a commercial sugarcane hybrid (Saccharum sp.). Plant Cell Rep 9: 419–423.Google Scholar
  6. Gnanapragasam S and Vasil IK (1992) Cryopreservation of immature embryos, embryogenic callus and cell suspension cultures of gramineous species. Plant Sci 83: 205–215.Google Scholar
  7. Kartha KK ed. (1985) Cryopreservation of Plant Cells and Organs, CRC Press, Boca Raton, Florida.Google Scholar
  8. Kartha KK, Fowke LC, Leung NL, Caswell KL and Hakman I (1988) Induction of somatic embryos and plantlets from cryopreserved cell cultures of white spruce (Picea glauca). J Plant Physiol 132: 529–539.Google Scholar
  9. Larkin PJ and Scowcroft WR (1981) Somaclonal variation — a novel source of variability from cell cultures for plant improvement. Theor Appl Genet 60: 197–214.Google Scholar
  10. Lee M and Phillips RL (1988) Chromosomal basis of somaclonal variation. Ann Rev Plant Physiol Plant Mol Biol 39: 413–437.Google Scholar
  11. Li R, Stelly DM and Trolinder NL (1989) Cytogenetic abnormalities in cotton (Gossypium hirsutum L.) cell cultures. Genome 32: 1128–1134.Google Scholar
  12. Murashige T and Skoog F (1962) A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol Plant 15: 473–497.Google Scholar
  13. Rangan TS (1993) Regeneration of cotton. US Patent 5,244,802.Google Scholar
  14. Rajasekaran K, Schank SC and Vasil IK (1986) Characterization of biomass production, cytology and phenotype of plant regenerated from embryogenic callus cultures of Pennisetum americanum x P. purpureum (hybrid triploid napiergrass). Theor Appl Genet 73: 4–10.Google Scholar
  15. Stelly DM, Altman DW, Kohel RJ, Rangan TS and Commiskey E (1989) Cytogenetic abnormalities of cotton somaclones from callus cultures. Genome 32: 762–770.Google Scholar
  16. Trolinder NT and Goodin JR (1987) Somatic embryogenesis and plant regeneration in cotton (Gossypium hirsutum L.). Plant Cell Rep 6: 231–234.Google Scholar
  17. Vasil IK ed. (1986) Cell Culture and Somatic Cell Genetics of Plants. Vol. 3, Plant Regeneration and Genetic Variability. Academic Press, Inc. Orlando, Florida.Google Scholar
  18. Widholm JM (1972) The use of flurescein diacetate and phenosafranine for determining viability of cultured plant cells. Stain Technol 47: 189–194.Google Scholar
  19. Withers LA (1985) Cryopreservation of cultured cells and protoplasts. In: Kartha KK (ed) Cryopreservation of Plant Cells and Organs, CRC Press, Boca Raton, Florida, pp. 243–267.Google Scholar

Copyright information

© Springer-Verlag 1996

Authors and Affiliations

  • Kanniah Rajasekaran
    • 1
  1. 1.J. G. Boswell CompanyCorcoranUSA

Personalised recommendations