Plant Cell Reports

, Volume 15, Issue 11, pp 859–864 | Cite as

Regeneration of plants from cryopreserved embryogenic cell suspension and callus cultures of cotton (Gossypium hirsutum L.)

  • Kanniah Rajasekaran


Successful regeneration of cotton (Gossypium hirsutum L.) plants from cryopreserved embryogenic callus and cell suspension cultures is described. The cryoprotectant mixture consisting of a modified Murashige and Skoog (1962) medium with sucrose (5% w/v), DMSO (5% v/v) and glycerol (5% v/v) gave the highest survival rate (70%) from cell suspension cultures cryopreserved in liquid nitrogen after slow cooling (0.5 to 1.0°C/min). A cooling rate of 0.5°C/min provided a satisfactory recovery rate (30%) from cryopreserved embryogenic callus cultures and was superior to a cooling rate of 1°C/min. Regenerated plants from cell suspension and embryogenic callus cultures cryopreserved for more than four years exhibited normal morphology, growth and boll set upon transfer to soil.


Sucrose Glycerol DMSO Cell Suspension Cooling Rate 
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Murashige and Skoog (1962)


modified MS


α-naphthaleneacetic acid


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Copyright information

© Springer-Verlag 1996

Authors and Affiliations

  • Kanniah Rajasekaran
    • 1
  1. 1.J. G. Boswell CompanyCorcoranUSA

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