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Plant Cell Reports

, Volume 15, Issue 11, pp 846–850 | Cite as

In vitro propagation of the medicinal herbs Ocimum americanum L. syn. O. canum Sims. (hoary basil) and Ocimum sanctum L. (holy basil)

  • Sitakanta Pattnaik
  • Pradeep K. Chand
Article

Abstract

A procedure is outlined for in vitro propagation of two medicinal herbs, Ocimum americanum L. syn. O. canum Sims (hoary basil) and Ocimum sanctum L. (holy basil), using axillary shoot buds. Multiple shoot formation was induced from shoot bud explants of both species on Murashige and Skoog medium (MS) supplemented with benzyladenine (BA). The optimum BA concentrations for shoot proliferation were 0.25 mg/l for O. americanum and 1.0 mg/l for O. sanctum. Incorporation of 0.5 mg/l gibberellic acid (GA3) along with BA in the culture medium resulted in a marked increase in the frequency of axillary branching as well as multiple shoot formation. Shoot buds collected between September through December were most responsive in culture. Shoots of O. americanum were rooted on half-strength MS supplemented with 1.0 mg/l indole-3-butyric acid (IBA), whereas O. sanctum rooted best on medium with 1.0 mg/l naphthaleneacetic acid (NAA). The plantlets were hardened off and successfully established in natural soil, where they grew and matured normally.

Keywords

Medicinal Herb Gibberellic Acid Natural Soil Benzyladenine Naphthaleneacetic Acid 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Abbreviations

BA

N6-benzyladenine

2,4-D

2,4-dichlorophenoxyacetic acid

GA3

gibberellic acid

IAA

indole-3-acetic acid

IBA

indole-3-butyric acid

MS

Murashige and Skoog (1962) medium

NAA

1-naphthaleneacetic acid

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References

  1. Ahuja A, Verma M, Grewal S (1982) Ind J Exp Biol 20: 455–458Google Scholar
  2. Arora R, Bhojwani SS (1989) Plant Cell Rep 8: 44–47Google Scholar
  3. Bajaj YPS, Furmanowa M, Olszowska O (1988) In: Bajaj YPS (ed) Biotechnology in agriculture and forestry, vol 4, Medicinal and aromatic plants I. Springer-Verlag, Berlin, Heidelberg, pp 60–103Google Scholar
  4. Earle ED, Langhans RW (1974) J Amer Soc Hort Sci 99: 128–132Google Scholar
  5. Heide OM (1969) Physiol Plant 22: 671–679Google Scholar
  6. Kukreja AK, Mathur AK (1985) Planta Med 2: 93–96Google Scholar
  7. Kukreja AK, Mathur AK, Zaim M (1990) Trop Agric 67: 101–104Google Scholar
  8. Murashige T, Skoog F (1962) Physiol Plant 15: 473–497Google Scholar
  9. Negrutiu I, Jacobs M, Cachita D (1978) Z Pflanzenphysiol 86: 113–124Google Scholar
  10. Nitsch C, Nitsch JP (1967) Planta 72: 355–370Google Scholar
  11. Pattnaik SK, Sahoo Y, Chand PK (1995) Scientia Hort 61: 227–239Google Scholar
  12. Purohit SD, Dave A, Kukda G (1994) Plant Cell Tiss Org Cult 39: 93–96Google Scholar
  13. Rech EL, Pires MJP (1986) Plant Cell Rep 5: 17–18Google Scholar
  14. Sen J, Sharma AK (1991) Plant Cell Tiss Org Cult 26: 71–73Google Scholar
  15. Sudha GC, Seeni S (1994) Plant Cell Rep 13: 203–207Google Scholar
  16. Thorpe TA, Murashige T (1970) Can J Bot 48: 277–285Google Scholar
  17. Wealth of India (1966) A dictionary of Indian raw materials and industrial products, vol VII (N-Pe). CSIR Publication, New Delhi, pp 79–89Google Scholar

Copyright information

© Springer-Verlag 1996

Authors and Affiliations

  • Sitakanta Pattnaik
    • 1
  • Pradeep K. Chand
    • 1
  1. 1.Plant Tissue and Cell Culture Facility, Post-Graduate Department of BotanyUtkal UniversityBhubaneswar — 751004, OrissaIndia

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