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Plant Cell Reports

, Volume 15, Issue 7, pp 489–494 | Cite as

Intron-mediated enhancement of gene expression in maize (Zea mays L.) and bluegrass (Poa pratensis L.)

  • Philippe Vain
  • Kim R. Finer
  • Dean E. Engler
  • Richard C. Pratt
  • John J. Finer
Article

Abstract

We report a strength comparison of a large variety of monocot and dicot intron-containing fragments inserted in the 5′ untranslated leader, between the CaMV 35S promoter and the uidA gene (coding for the ß-glucuronidase: GUS). Relative strengths of the intron-containing fragments were evaluated by comparing transient GUS expression after particle bombardment in embryogenic maize and bluegrass suspension cultures. Our results confirm a dramatic dependence on the presence of an intron for chimeric gene expression in both species. On average, the maize first intron of ubi1 provided the highest enhancement of gene expression in maize and bluegrass (71- and 26-fold enhancement, respectively). Half of the introns tested affected gene expression differently in bluegrass and maize. This suggests that the intron-mediated enhancement of gene expression generally obtained with maize may not be fully applicable to all monocots. We also report enhancement of gene expression (92-fold) in a monocot species by a dicot intron (chsA intron).

Keywords

Suspension Culture Particle Bombardment Chimeric Gene Affect Gene Expression uidA Gene 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Springer-Verlag 1996

Authors and Affiliations

  • Philippe Vain
    • 1
  • Kim R. Finer
    • 2
  • Dean E. Engler
    • 3
  • Richard C. Pratt
    • 1
  • John J. Finer
    • 1
  1. 1.Horticulture and Crop Science DepartmentThe Ohio State University, The Ohio Agricultural Research and Development CenterWoosterUSA
  2. 2.Department of Biological SciencesKent State University, Stark CampusCantonUSA
  3. 3.DNAPOaklandUSA

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