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Plant Cell Reports

, Volume 14, Issue 10, pp 666–669 | Cite as

Screening for in vitro shoot-forming capacity of seedling explants in bell pepper (Capsicum annuum L.) genotypes and efficient plant regeneration using thidiazuron

  • Attila Szász
  • Giuseppe Nervo
  • Miklós Fári
Article

Summary

In vitro shoot regeneration ability of 17 (7 Italian and 10 Hungarian) bell pepper genotypes was investigated using excised cotyledons and rooted hypocotyls as explants. Most of the Italian genotypes and two of the Hungarian genotypes responded well, producing shoots from rooted hypocotyls. Only two genotypes (one Italian and one Hungarian) gave a weak response using cotyledons. For direct shoot induction in these explants, in addition to the methods cited in the relevant papers, a new method was applied using thidiazuron as a cytokinin. Shoots were successfully regenerated from cotyledons of two Italian and two Hungarian genotypes using thidiazuron which were considered to be non responsive to the usual methods.

Keywords

Thidiazuron Cotyledon Explants Bell Pepper Pepper Cultivar Shoot Elongation Medium 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Abbreviations

BAP

6-benzylaminopurine

GA3

gibberellic acid

IAA

indole-3-acetic acid

MS medium

(Murashige and Skoog 1962)

TDZ

N-phenyl-N-thiadiazol-l,2,3-5-ylurea (thidiazuron)

PVC

polyvinyl chloride

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Copyright information

© Springer-Verlag 1995

Authors and Affiliations

  • Attila Szász
    • 1
  • Giuseppe Nervo
    • 2
  • Miklós Fári
    • 1
  1. 1.Agricultural Biotechnology CenterHungary
  2. 2.Research Institute for Vegetable CropsMontanasoItaly

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