In Spathiphyllum floribundum ‘Petite’, which was cultured on medium containing benzyladenine (BA), uptake of this cytokinin and its conversion to 9-ß-D-ribofuranosyl-benzyladenine (9R-BA) or 9-ß-glucopyranosyl-benzyladenine (9G-BA) was monitored. BA and extremely large quantities of 9G-BA were exclusively located in the basal part of the plant (callus and meristems). 9R-BA was found in the basal part, the petioles and the leaf blades. After an acclimatisation period of 9 weeks the plants still contained high levels of 9G-BA, but BA and 9R-BA could no longer be detected after one week. The possible role of BA and its derivatives on inhibition of root initiation or irreversible chloroplast deficiency is discussed.
Basal Part Leaf Blade Diode Array Detector Fresh Mass Benzyladenine
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high-performance liquid chromatography
liquid chromatography—mass spectrometry
photo-synthetic active radiation
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Biondi S, Canciani L (1984) Uptake and translocation of benzyladenine by elm shoots cultured in vitro. Can J Bot 62: 2385–2390CrossRefGoogle Scholar
Blakesley D (1991) Uptake and metabolism of 6-benzyladenine in shoot cultures of Musa and Rhododendron. Plant Cell, Tissue and Organ Culture 25:69–74CrossRefGoogle Scholar
Blakesley D, Constantine (1992) Uptake and metabolism of 6-benzyladenine in shoot cultures of a range of species. Plant Cell, Tissue and Organ Culture 28:183–186CrossRefGoogle Scholar
De Proft MP, Maene LJ, Debergh PC (1985) Carbon dioxide and ethylene evolution in the culture atmosphere of Magnolia cultured in vitro. Physiol Plant 65:375–379CrossRefGoogle Scholar
Minocha SC, Nissen P (1982) Uptake of benzyladenine by tuber slices of Jerusalem artichoke (Helianthus tuberosus L.) over a wide concentration range. Plant Physiol 70:528–531CrossRefPubMedPubMedCentralGoogle Scholar
Murashige T, Skoog F (1962) A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol Plant 15:473–497CrossRefGoogle Scholar
Nicander B, Ståhl U, Björkman P, Tillberg E (1993) Immuno affinity co-purification of cytokinins and analysis by high-performance liquid chromatography with ultraviolet-spectrum detection. Planta 189:312–320CrossRefPubMedGoogle Scholar
Nordström A, Eliasson L (1986) Uptake and translocation of [14C]-labelled benzylaminopurine in apple shoots grown in vitro in realtion to shoot development. Physiol Plant 68:431–435CrossRefGoogle Scholar
Vogelmann TC, Bornman CH, Nissen P (1984) Uptake of benzyladenine in explants of Picea abies and Pinus sylvestris. Physiol Plant 61:513–517CrossRefGoogle Scholar