Skip to main content
SpringerLink
Log in

The metabolism of benzyladenine in Spathiphyllum floribundum ‘Schott Petite’ in relation to acclimatisation problems

  • Published:
Plant Cell Reports Aims and scope Submit manuscript

Abstract

In Spathiphyllum floribundum ‘Petite’, which was cultured on medium containing benzyladenine (BA), uptake of this cytokinin and its conversion to 9-ß-D-ribofuranosyl-benzyladenine (9R-BA) or 9-ß-glucopyranosyl-benzyladenine (9G-BA) was monitored. BA and extremely large quantities of 9G-BA were exclusively located in the basal part of the plant (callus and meristems). 9R-BA was found in the basal part, the petioles and the leaf blades. After an acclimatisation period of 9 weeks the plants still contained high levels of 9G-BA, but BA and 9R-BA could no longer be detected after one week. The possible role of BA and its derivatives on inhibition of root initiation or irreversible chloroplast deficiency is discussed.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

Abbreviations

3G-BA:

3-ß-glucopyranosyl-benzyladenine

7G-BA:

7-ß-glucopyranosyl-benzyladenine

9G-BA:

9-ß-glucopyranosyl-benzyladenine

9R-BA:

9-ß-D-ribofuranosyl-benzyladenine

au:

absorption units

HPLC:

high-performance liquid chromatography

LC-MS:

liquid chromatography—mass spectrometry

MeOH:

methanol

NaFeEDTA:

ethylenediaminetetra-acetic acid

NH4Ac:

ammonium acetate

PAR:

photo-synthetic active radiation

UV:

ultra-violet

References

  • Biondi S, Canciani L (1984) Uptake and translocation of benzyladenine by elm shoots cultured in vitro. Can J Bot 62: 2385–2390

    Article  CAS  Google Scholar 

  • Blakesley D (1991) Uptake and metabolism of 6-benzyladenine in shoot cultures of Musa and Rhododendron. Plant Cell, Tissue and Organ Culture 25:69–74

    Article  CAS  Google Scholar 

  • Blakesley D, Constantine (1992) Uptake and metabolism of 6-benzyladenine in shoot cultures of a range of species. Plant Cell, Tissue and Organ Culture 28:183–186

    Article  CAS  Google Scholar 

  • De Proft MP, Maene LJ, Debergh PC (1985) Carbon dioxide and ethylene evolution in the culture atmosphere of Magnolia cultured in vitro. Physiol Plant 65:375–379

    Article  Google Scholar 

  • Minocha SC, Nissen P (1982) Uptake of benzyladenine by tuber slices of Jerusalem artichoke (Helianthus tuberosus L.) over a wide concentration range. Plant Physiol 70:528–531

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Murashige T, Skoog F (1962) A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol Plant 15:473–497

    Article  CAS  Google Scholar 

  • Nicander B, Ståhl U, Björkman P, Tillberg E (1993) Immuno affinity co-purification of cytokinins and analysis by high-performance liquid chromatography with ultraviolet-spectrum detection. Planta 189:312–320

    Article  CAS  PubMed  Google Scholar 

  • Nitsch JP, Nitsch C (1969) Haploid plants from pollen grains. Science 163:85–87

    Article  CAS  PubMed  Google Scholar 

  • Nordström A, Eliasson L (1986) Uptake and translocation of [14C]-labelled benzylaminopurine in apple shoots grown in vitro in realtion to shoot development. Physiol Plant 68:431–435

    Article  Google Scholar 

  • Vogelmann TC, Bornman CH, Nissen P (1984) Uptake of benzyladenine in explants of Picea abies and Pinus sylvestris. Physiol Plant 61:513–517

    Article  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of Plant Production, Horticulture University Gent, Coupure links 653, 9000, Gent, Belgium

    Stefaan P. O. Werbrouck, Bruno van der Jeugt & Pierre C. Debergh

  2. Department of Biology, U.I.A., 2610, Wilrijk, Belgium

    Walter Dewitte, Els Prinsen & Harry A. Van Onckelen

Authors
  1. Stefaan P. O. Werbrouck
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Bruno van der Jeugt
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Walter Dewitte
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Els Prinsen
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Harry A. Van Onckelen
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Pierre C. Debergh
    View author publications

    You can also search for this author in PubMed Google Scholar

Additional information

Communicated by A. M. Boudet

Rights and permissions

Reprints and permissions

About this article

Cite this article

Werbrouck, S.P.O., van der Jeugt, B., Dewitte, W. et al. The metabolism of benzyladenine in Spathiphyllum floribundum ‘Schott Petite’ in relation to acclimatisation problems. Plant Cell Reports 14, 662–665 (1995). https://doi.org/10.1007/BF00232734

Download citation

  • Received:

  • Revised:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF00232734

Keywords

Search

Navigation