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Plant Cell Reports

, Volume 14, Issue 10, pp 662–665 | Cite as

The metabolism of benzyladenine in Spathiphyllum floribundum ‘Schott Petite’ in relation to acclimatisation problems

  • Stefaan P. O. Werbrouck
  • Bruno van der Jeugt
  • Walter Dewitte
  • Els Prinsen
  • Harry A. Van Onckelen
  • Pierre C. Debergh
Article

Abstract

In Spathiphyllum floribundum ‘Petite’, which was cultured on medium containing benzyladenine (BA), uptake of this cytokinin and its conversion to 9-ß-D-ribofuranosyl-benzyladenine (9R-BA) or 9-ß-glucopyranosyl-benzyladenine (9G-BA) was monitored. BA and extremely large quantities of 9G-BA were exclusively located in the basal part of the plant (callus and meristems). 9R-BA was found in the basal part, the petioles and the leaf blades. After an acclimatisation period of 9 weeks the plants still contained high levels of 9G-BA, but BA and 9R-BA could no longer be detected after one week. The possible role of BA and its derivatives on inhibition of root initiation or irreversible chloroplast deficiency is discussed.

Keywords

Basal Part Leaf Blade Diode Array Detector Fresh Mass Benzyladenine 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Abbreviations

3G-BA

3-ß-glucopyranosyl-benzyladenine

7G-BA

7-ß-glucopyranosyl-benzyladenine

9G-BA

9-ß-glucopyranosyl-benzyladenine

9R-BA

9-ß-D-ribofuranosyl-benzyladenine

au

absorption units

HPLC

high-performance liquid chromatography

LC-MS

liquid chromatography—mass spectrometry

MeOH

methanol

NaFeEDTA

ethylenediaminetetra-acetic acid

NH4Ac

ammonium acetate

PAR

photo-synthetic active radiation

UV

ultra-violet

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Copyright information

© Springer-Verlag 1995

Authors and Affiliations

  • Stefaan P. O. Werbrouck
    • 1
  • Bruno van der Jeugt
    • 1
  • Walter Dewitte
    • 2
  • Els Prinsen
    • 2
  • Harry A. Van Onckelen
    • 2
  • Pierre C. Debergh
    • 1
  1. 1.Department of Plant ProductionHorticulture University GentGentBelgium
  2. 2.Department of BiologyU.I.A.WilrijkBelgium

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