Plant Cell Reports

, Volume 10, Issue 8, pp 394–396 | Cite as

Effect of calcium, manganese and lithium on growth and cardenolide content in cell suspension cultures of Digitalis thapsi L.

  • M. Purificación Corchete
  • M. Amparo Jimenez
  • Margarita Moran
  • Margarita Cacho
  • Jorge Fernandez-Tarrago
Article

Abstract

Cell suspension cultures of Digitalis thapsi were grown in Murashige and Skoog medium under continuous light. The effects of the absence of CaCl2, elevation of the MnSO4 concentration from 0.1 mM to 5 mM or the addition of 100 μM LiCl on their growth and digoxin production were investigated. The elimination of calcium reduced growth and viability of cultures but promoted digoxin formation. An increase of the MnSO4 concentration or the addition of LiCl resulted in higher digoxin content. Under such conditions growth was not affected.

Abbreviations

2,4-D

2,4-dichlorophenoxyacetic acid

BA

N6 benzyladenine

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References

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Copyright information

© Springer-Verlag 1991

Authors and Affiliations

  • M. Purificación Corchete
    • 1
  • M. Amparo Jimenez
    • 1
  • Margarita Moran
    • 1
  • Margarita Cacho
    • 1
  • Jorge Fernandez-Tarrago
    • 1
  1. 1.Departamento de Biología VegetalLaboratorio de Fisiología Vegetal, Facultad de Farmacia, Universidad de SalamancaSalamancaSpain

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