Plant Cell Reports

, Volume 15, Issue 8, pp 610–614 | Cite as

Increase of root induction in Pinus nigra explants using agrobacteria

  • Snježana Mihaljević
  • Suzana Stipković
  • Sibila Jelaska
Article
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Revised:

Summary

Wounding of explanted Pinus nigra primary explants followed by infection with Agrobacterium rhizogenes wild strains 8196, 15834, or with the pRiA4abc transconjugant strain of A. tumefaciens (C58 chromosomal background) resulted in adventitious root induction. Roots were formed in 60–97% of explants (1–3 roots/explant) but without a hairy root phenotype. The presence of T-DNA of pRi8196 or pRiA4abc in regenerated roots was confirmed by the opine (mannopinic acid) content. Transformation response was influenced by the bacterial strain, age of explant and period of co-cultivation. Both the aggregate state (liquid) of medium and the season of the year (spring) had a positive effect on the root induction and their development. Histological analysis of the transformed roots showed that complete elements of primary and secondary root structures were present but roots were always triarch or tetrarch in the central cylinder as opposed to the primary roots of the untransformed seedling wich are diarch.

Keywords

Hairy Root Adventitious Root Primary Root Root Induction Wild Strain 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.
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Copyright information

© Springer-Verlag 1996

Authors and Affiliations

  • Snježana Mihaljević
    • 1
  • Suzana Stipković
    • 1
  • Sibila Jelaska
    • 1
  1. 1.Faculty of Science, Department of Molecular BiologyInstitute Ruder Bošković, Bijenička 54, and University of ZagrebZagrebCroatia

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