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Plant Cell Reports

, Volume 15, Issue 8, pp 578–581 | Cite as

Adventitious shoot regeneration from leaf, stem and root explants of commercial cultivars of Gentiana

  • Keizo Hosokawa
  • Masaru Nakano
  • Yayoi Oikawa
  • Saburo Yamamura
Article

Abstract

Several culture conditions were examined for promoting efficient plant regeneration from explants of Gentiana. Adventitious shoot regeneration from leaf explants of cv. WSP-3 was very superior on MS medium, compared to B5 medium, supplemented with four cytokinins (TDZ, 4PU-30, BA and zeatin). An auxin / cytokinin combination was required for regeneration. TDZ was the most effective cytokinin, while NAA was more effective than IAA or 2,4-D. Optimum conditions for regeneration from explants (leaf, stem and root) of cv. WSP-3, evaluated in terms of regeneration frequency and number of regenerated shoots per explant, were TDZ and NAA in combination, 5–10 mg/l and 0.1 mg/l for leaf and stem explants, and 10 mg/l and 1 mg/l for root explants, respectively. Application of these conditions to eight other commercial cultivars resulted in 30–100% regeneration from leaf explants. The number of chromosomes in each of ten regenerated plants of each cultivar was diploid, 2n=26. Shoots regenerated in vitro were rooted in phytohormone-free medium and transferred to soil.

Key words

Gentiana plant regeneration TDZ 

Abbreviations

MS medium

Murashige and Skoog's medium (Murashige and Skoog 1962)

B5 medium

Gamborg B5 medium (Gamborg et al. 1968)

BA

6-benzylaminopurine

TDZ

N-phenyl-N'-1,2,3-thiadiazol-5-yl urea

4PU-30

N-(2-chloro-4-pyridyl)-N'-phenylurea

2,4-D

2,4-dichlorophenoxyacetic acid

IAA

indole-3-acetic acid

NAA

1-naphthaleneacetic acid

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Copyright information

© Springer-Verlag 1996

Authors and Affiliations

  • Keizo Hosokawa
    • 1
  • Masaru Nakano
    • 1
  • Yayoi Oikawa
    • 1
  • Saburo Yamamura
    • 1
  1. 1.Iwate Biotechnology Research CenterIwateJapan

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