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Plant Cell Reports

, Volume 15, Issue 8, pp 561–565 | Cite as

Stress-induced microspore embryogenesis in tobacco: an optimized system for molecular studies

  • Alisher Touraev
  • Andi Ilham
  • Oscar Vicente
  • Erwin Heberle-Bors
Article

Summary

Specific stress treatments applied to isolated tobacco (Nicotiana tabacum L.) microspores efficiently induced haploid embryo formation in vitro. A heat shock at 33 or 37°C in the presence of sugar, as well as sucrose-starvation at 25°C, resulted in the formation of embryogenic microspores. A combination of both treatments had an additive effect. Under optimal induction conditions all viable microspores in the culture were embryogenic and developed subsequently into pollen embryos by culture at 25°C in a sugar-containing medium, with induction frequencies of more than 70% with respect to the initial microspore population. A high fraction of the early pollen embryos continued their development in vitro, giving rise to haploid plants. In contrast to other available systems for microspore/pollen embryogenesis, the new protocol allows the production of homogeneous populations of embryogenic microspores and early globular embryos in large-scale cultures, without any purification step, and is therefore well suited for biochemical and molecular work.

Keywords

Embryo Formation Haploid Plant Induction Frequency Globular Embryo Microspore Embryogenesis 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Abbreviations

EDTA

ethylenediaminetetraacetate

DAPI

4,6-diamidino-2-phenylindole

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Copyright information

© Springer-Verlag 1996

Authors and Affiliations

  • Alisher Touraev
    • 1
  • Andi Ilham
    • 1
  • Oscar Vicente
    • 1
  • Erwin Heberle-Bors
    • 1
  1. 1.Vienna Biocenter, Institute of Microbiology and GeneticsUniversity of ViennaViennaAustraia

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