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The Journal of Membrane Biology

, Volume 139, Issue 2, pp 127–136 | Cite as

Regulation of gap junctional coupling in isolated pancreatic acinar cell pairs by cholecystokinin-octapeptide, vasoactive intestinal peptide (VIP) and a VIP-antagonist

  • A. Ngezahayo
  • H. -A. Kolb
Article

Abstract

Cholecystokinin-octapeptide (CCK-OP) induces a time- and dose-dependent decrease of gap Junctional conductance in isolated pairs of pancreatic acinar cells. In double whole-cell experiments, the time course could be described by the latency and the half-life time (t 1/2 ) of cell-to-cell uncoupling. The latency shows a biphasic dependence on [CCK-OP] with a minimum of about 50 sec at 10−9m CCK-OP. In the presence of vasoactive intestinal peptide (VIP), the biphasic relationship is shifted to lower CCK-OP concentrations. The increase of latency at high concentrations of CCK-OP (> 1009m) was blocked by addition of a VIP-antagonist. t 1/2 decreases monophasically with increasing [CCKOP]. Addition of GTPγS to the pipette solution suppresses the [CCK-OP] dependence of the latency and potentiates the uncoupling phase. The kinetic data are discussed in terms of CCK binding to receptors of high and low affinity. Evidence is presented that secretion and cell-to-cell coupling are not related by an all-ornone process, but that for physiological CCK-OP concentrations, gap junctional uncoupling follows secretion.

Key words

Gap junctions Double whole cell Cholecystokinin-octapeptide Vasoactive intestinal peptide (VIP) VIP antagonist Pancreatic acinar cells 

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Copyright information

© Springer-Verlag New York Inc 1994

Authors and Affiliations

  • A. Ngezahayo
    • 1
  • H. -A. Kolb
    • 2
  1. 1.University of Konstanz, Faculty of BiologyKonstanzGermany
  2. 2.University of Tübingen, Institut of PhysiologyTübingenGermany

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