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Plant Cell Reports

, Volume 9, Issue 7, pp 398–401 | Cite as

Plant regeneration by somatic embryogenesis from cultured immature embryos of oak (Querem robur L.) and linden (Tilia cordata Mill.)

  • Vladimir Chalupa
Article

Abstract

Embryogenic cultures and somatic embryos were obtained from immature zygotic embryos of oak (Quercus robur L.) cultured on a modified MS medium and WPM containing BAP (1 mg·l−1) and GA3 (1 mg·l−1) or BAP and IBA. Germination and conversion of oak somatic embryos into plantlets was achieved on WPM containing a reduced concentration of cytokinin. Linden (Tilia cordata Mill.) somatic embryos developed in embryogenic tissues initiated from immature zygotic embryos cultured on a modified MS medium supplemented with 2,4-D (0.3-2.0 mg·l−1). Germination of linden somatic embryos and plantlet formation occurred on MS medium containing a low concentration of IBA. Oak and linden plantlets produced from somatic embryos were successfully established in soil. Somatic embryos and plantlets were also regenerated from embryogenic cultures of Quercus petraea and Tilia platyphyllos.

Keywords

Somatic Embryo Somatic Embryogenesis Plant Regeneration Zygotic Embryo Immature Embryo 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Abbreviations

BAP

6-benzyIaminopurine

GA3

gibberellic acid

IBA

indole-3-butyric acid

2,4-D

2,4-dichlorophenoxyacetic acid

MS

Murashige and Skoog (1962)

WPM

woody plant medium

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References

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Copyright information

© Springer-Verlag 1990

Authors and Affiliations

  • Vladimir Chalupa
    • 1
  1. 1.Forestry and Game Management Research InstituteZbraslav-Strnady

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