Plant Cell Reports

, Volume 9, Issue 10, pp 590–594

Stable transformation of Populus and incorporation of pest resistance by electric discharge particle acceleration

  • B. H. McCown
  • D. E. McCabe
  • D. R. Russell
  • D. J. Robison
  • K. A. Barton
  • K. F. Raffa
Article

DOI: 10.1007/BF00232339

Cite this article as:
McCown, B.H., McCabe, D.E., Russell, D.R. et al. Plant Cell Reports (1991) 9: 590. doi:10.1007/BF00232339

Abstract

Three different target tissues (protoplast-derived cells, nodules, and stems) and two unrelated hybrid genotypes of Populus (P. alba x P. grandidentata ‘Crandon’ and P. nigra ‘Betulifolia’ x P. trichocarpa) have been stably transformed by electric discharge particle acceleration using a 18.7 kb plasmid containing NOS-NPT, CaMV 35S-GUS, and CaMV 35S-BT. Four transformed plants of one hybrid genotype, NC5339, containing all 3 genes were recovered and analyzed. Two expressed GUS and one was highly resistant to feeding by 2 lepidopteran pests (the forest tent caterpillar, Malacosoma disstria, and the gypsy moth, Lymantria dispar.) Pretreatment of the target tissues, fine-tuning of the bombardment parameters, and the use of a selection technique employing flooding of the target tissues were important for reliable recovery of transformed plants.

Abbreviations

BA

benzyladenine

NAA

1-naphthaleneacetic acid

TDZ

thidiazuron

DB

2,6-dichlorobenzonitrile

WPM

woody plant medium

Kan

kanamycin

Carb

carbenicillin

Ben

benylate

gus

β-glucuronidase (EC 3.2.1.31)

NPT

neomycin phosphoransferase (EC 2.7.1.95) gene

GUS

betaglucuronidase gene

BT

modified endotoxin gene originally from Bacillus thuringiensis (B.t.)

PCR

polymerase chain reaction

Copyright information

© Springer-Verlag 1991

Authors and Affiliations

  • B. H. McCown
    • 1
  • D. E. McCabe
    • 3
  • D. R. Russell
    • 3
  • D. J. Robison
    • 2
  • K. A. Barton
    • 3
  • K. F. Raffa
    • 2
  1. 1.Department of HorticultureUniversity of Wisconsin-MadisonMadisonUSA
  2. 2.Department of EntomologyUniversity of Wisconsin-MadisonMadisonUSA
  3. 3.AgracetusMiddletonUSA

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