Plant Cell Reports

, Volume 9, Issue 10, pp 590–594 | Cite as

Stable transformation of Populus and incorporation of pest resistance by electric discharge particle acceleration

  • B. H. McCown
  • D. E. McCabe
  • D. R. Russell
  • D. J. Robison
  • K. A. Barton
  • K. F. Raffa


Three different target tissues (protoplast-derived cells, nodules, and stems) and two unrelated hybrid genotypes of Populus (P. alba x P. grandidentata ‘Crandon’ and P. nigra ‘Betulifolia’ x P. trichocarpa) have been stably transformed by electric discharge particle acceleration using a 18.7 kb plasmid containing NOS-NPT, CaMV 35S-GUS, and CaMV 35S-BT. Four transformed plants of one hybrid genotype, NC5339, containing all 3 genes were recovered and analyzed. Two expressed GUS and one was highly resistant to feeding by 2 lepidopteran pests (the forest tent caterpillar, Malacosoma disstria, and the gypsy moth, Lymantria dispar.) Pretreatment of the target tissues, fine-tuning of the bombardment parameters, and the use of a selection technique employing flooding of the target tissues were important for reliable recovery of transformed plants.


Alba Electric Discharge Target Tissue Particle Acceleration Selection Technique 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.





1-naphthaleneacetic acid






woody plant medium








β-glucuronidase (EC


neomycin phosphoransferase (EC gene


betaglucuronidase gene


modified endotoxin gene originally from Bacillus thuringiensis (B.t.)


polymerase chain reaction


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. Barton KA, Whiteley HR, Yang N (1987) Plant Physiol 85:1103–1109.Google Scholar
  2. Bekkaoui F, Pilon M, Laine E, Raju DSS, Crosby WL, Dunstan DI (1988) Plant Cell Rep 7:481–484.Google Scholar
  3. Beremand PD, Hannapel DJ, Guerra DJ, Kuhn DN, Ohlrogge JB (1987) Arch Biochem Biophys 256:90–100.Google Scholar
  4. Christou P, Swain W, Yang N.-S, McCabe D (1989) PNAS 86:7500–7504.Google Scholar
  5. Dellaporta S, Wood J, Hicks J (1983) Plant Mol Biol Rep 1:19–21.Google Scholar
  6. Fillatti JJ, Sellmer J, McCown B, Haissig B, Comai L. (1987) Mol Gen Genet 206:192–199.Google Scholar
  7. Fischhoff DA, Bowdisk KS, Perlak FJ, Marrone PG, McCormick SM, Niedermeyer JG, Dean DA, Kusano-Kretzner K, Meyer FJ, Rochester DE, Rogers SG, Fraley RT (1987) Bio/Technology 5:807–813.Google Scholar
  8. Galbraith DW, Shields BA (1982) Physiol Plant 55:25–30.Google Scholar
  9. Grisdale D (1985) In: Singh P, Moore RS (eds), Handbook of Insect Rearing, Vol. II, Elsevier Publishing, NY, pp. 369–379.Google Scholar
  10. Heimpel AM, Angus TA (1959) J Ins Path 1:152–170.Google Scholar
  11. James DJ, Passey, AJ, Barbara DJ, Bevan M (1989) Plant Cell Rep 7:658–661.Google Scholar
  12. Jefferson RA, Kavanagh TA, Bewan MW (1987) EMBO J 6:3901–3907.PubMedGoogle Scholar
  13. Krieg A, Langenbruch GA (1981) In: H.D. Burgess HD (ed.), Microbial Control of Pests and Plant Diseases, 1970–1980, Academic Press, N.Y. pp. 837–1097.Google Scholar
  14. Lloyd G, McCown B (1980) Proc Int Plant Prop Soc 30:420–427.Google Scholar
  15. McCabe DE, Swain WF, Martinell BJ, Christou P. (1988) Bio/Technology 6:923–926.Google Scholar
  16. McCown BH, Zeldin E, Pinkalla H, Dedolph R (1988) In: Hanover J, Keathley D (eds), Genetic Manipulation of Woody Plants, Plenum Press, Seattle, WA, pp. 149–166.Google Scholar
  17. McGranahan G, Leslie C, Uratsu S, Martin L, Dandekar A (1988) Bio/Technology 6:800–804.Google Scholar
  18. Montgomery ME, Wallner WE (1988) In: Berryman AA (ed.), Dynamics of Forest Insect Populations, Plenum Press, N.Y., pp. 354–375.Google Scholar
  19. Murray M, Thompson W (1980) Nucl Acids Res 8:4321–4325.Google Scholar
  20. Pythoud F, Sinkar VP, Nester EW, Gordon MP (1987) Bio/Technology 5:1323–1327.Google Scholar
  21. Raffa KF (1989) BioScience 39:524–534.Google Scholar
  22. Ranney JW, Wright LL, Layton PA (1987) J For 85:17–28.Google Scholar
  23. Russell JA, McCown BH (1988) Plant Cell Rep 7:59–62.Google Scholar
  24. Saiki R, Gelfaud D, Stoffel S, Scharf S, Higuchi R, Horn G, Mullis K, Erlich, H (1988) Science 239:487–494.PubMedGoogle Scholar
  25. SAS (1982) SAS Users Guide: Statistics. SAS Institute, Cary, NC.Google Scholar
  26. Schnepf HE, Wong HC, Whiteley HR (1985) J Biol Chem 260:6264–6272.Google Scholar
  27. Seguin A, Lalonde M (1988) Plant Cell Rep 7:367–370.Google Scholar
  28. Sellmer JC, McCown BH (1989) In: Bajaj YPS (ed) Plant Protoplasts and Genetic Engineering II, Springer Verlag, Berlin. pp. 155–172.Google Scholar
  29. Sellmer JC, McCown BH, Haissig BE (1989) Tree Physiol 5:219–227.Google Scholar
  30. Vaeck M, Reynaerts A, Hofte H, Jensens S, DeBeuckeleer M, Dean C, Abeau M, VanMontagu M, Leemand J (1987) Nature 328:33–37.Google Scholar

Copyright information

© Springer-Verlag 1991

Authors and Affiliations

  • B. H. McCown
    • 1
  • D. E. McCabe
    • 3
  • D. R. Russell
    • 3
  • D. J. Robison
    • 2
  • K. A. Barton
    • 3
  • K. F. Raffa
    • 2
  1. 1.Department of HorticultureUniversity of Wisconsin-MadisonMadisonUSA
  2. 2.Department of EntomologyUniversity of Wisconsin-MadisonMadisonUSA
  3. 3.AgracetusMiddletonUSA

Personalised recommendations