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Plant Cell Reports

, Volume 9, Issue 10, pp 590–594 | Cite as

Stable transformation of Populus and incorporation of pest resistance by electric discharge particle acceleration

  • B. H. McCown
  • D. E. McCabe
  • D. R. Russell
  • D. J. Robison
  • K. A. Barton
  • K. F. Raffa
Article

Abstract

Three different target tissues (protoplast-derived cells, nodules, and stems) and two unrelated hybrid genotypes of Populus (P. alba x P. grandidentata ‘Crandon’ and P. nigra ‘Betulifolia’ x P. trichocarpa) have been stably transformed by electric discharge particle acceleration using a 18.7 kb plasmid containing NOS-NPT, CaMV 35S-GUS, and CaMV 35S-BT. Four transformed plants of one hybrid genotype, NC5339, containing all 3 genes were recovered and analyzed. Two expressed GUS and one was highly resistant to feeding by 2 lepidopteran pests (the forest tent caterpillar, Malacosoma disstria, and the gypsy moth, Lymantria dispar.) Pretreatment of the target tissues, fine-tuning of the bombardment parameters, and the use of a selection technique employing flooding of the target tissues were important for reliable recovery of transformed plants.

Keywords

Alba Electric Discharge Target Tissue Particle Acceleration Selection Technique 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Abbreviations

BA

benzyladenine

NAA

1-naphthaleneacetic acid

TDZ

thidiazuron

DB

2,6-dichlorobenzonitrile

WPM

woody plant medium

Kan

kanamycin

Carb

carbenicillin

Ben

benylate

gus

β-glucuronidase (EC 3.2.1.31)

NPT

neomycin phosphoransferase (EC 2.7.1.95) gene

GUS

betaglucuronidase gene

BT

modified endotoxin gene originally from Bacillus thuringiensis (B.t.)

PCR

polymerase chain reaction

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Copyright information

© Springer-Verlag 1991

Authors and Affiliations

  • B. H. McCown
    • 1
  • D. E. McCabe
    • 3
  • D. R. Russell
    • 3
  • D. J. Robison
    • 2
  • K. A. Barton
    • 3
  • K. F. Raffa
    • 2
  1. 1.Department of HorticultureUniversity of Wisconsin-MadisonMadisonUSA
  2. 2.Department of EntomologyUniversity of Wisconsin-MadisonMadisonUSA
  3. 3.AgracetusMiddletonUSA

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