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Plant Cell Reports

, Volume 9, Issue 10, pp 563–566 | Cite as

Regeneration of multiple shoots and plants from Mesembryanthemum crystallinum

  • Michael S. Meiners
  • John C. Thomas
  • Hans J. Bohnert
  • John C. Cushman
Article

Abstract

Mesembryanthemum crystallinum plants have been regenerated via organogenesis from hypocotyl, cotyledonary node, and leaf expiants with varying frequencies. The highest regeneration frequencies were obtained from either hypocotyls (23–34%) or cotyledonary nodes (21–41%). Leaf expiants yielded very poor regeneration frequencies (0–11%). Expiants were placed on Murashige and Skoog (MS) media supplemented with 3% sucrose, 0.8% bacto-agar and either, 10.8×10−6M NAA and 8.8×10−6M BA (MSmsh), 1×10−5M BA and 1×10−6M IAA, (MS4) or 1×10−6M BA and 1×10−6M IAA (MS5). Shoot formation frequencies were greater on MS4 and MS5 and lower on MSmsh, however, overall differences of regeneration frequency among media tested were not statistically significant. Regenerated plantlets were rooted on MS medium without growth regulators. Mature, regenerated plants were fertile and exhibited DNA content and ploidy profiles that were identical to wild type plants.

Keywords

Sucrose Growth Regulator Regenerate Plant Wild Type Plant Regeneration Frequency 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Abbreviations

MS

Murashige and Skoog media

CAM

Crassulacean acid metabolism

kbp

kilobase pairs

NAA

1-naphthaleneacetic acid

BA

6-benzyladenine

IAA

indole-3-acetic acid

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References

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Copyright information

© Springer-Verlag 1991

Authors and Affiliations

  • Michael S. Meiners
    • 1
  • John C. Thomas
    • 1
  • Hans J. Bohnert
    • 1
  • John C. Cushman
    • 1
  1. 1.Department of BiochemistryThe University of Arizona, BioSciences WestTucsonUSA

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