Regeneration of multiple shoots and plants from Mesembryanthemum crystallinum
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Mesembryanthemum crystallinum plants have been regenerated via organogenesis from hypocotyl, cotyledonary node, and leaf expiants with varying frequencies. The highest regeneration frequencies were obtained from either hypocotyls (23–34%) or cotyledonary nodes (21–41%). Leaf expiants yielded very poor regeneration frequencies (0–11%). Expiants were placed on Murashige and Skoog (MS) media supplemented with 3% sucrose, 0.8% bacto-agar and either, 10.8×10−6M NAA and 8.8×10−6M BA (MSmsh), 1×10−5M BA and 1×10−6M IAA, (MS4) or 1×10−6M BA and 1×10−6M IAA (MS5). Shoot formation frequencies were greater on MS4 and MS5 and lower on MSmsh, however, overall differences of regeneration frequency among media tested were not statistically significant. Regenerated plantlets were rooted on MS medium without growth regulators. Mature, regenerated plants were fertile and exhibited DNA content and ploidy profiles that were identical to wild type plants.
KeywordsSucrose Growth Regulator Regenerate Plant Wild Type Plant Regeneration Frequency
Murashige and Skoog media
Crassulacean acid metabolism
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