Plant Cell Reports

, Volume 9, Issue 10, pp 544–548

Characterization of a maize endosperm culture expressing zein genes and its use in transient transformation assays

  • Tom J. A. Quayle
  • Winfried Hetz
  • Günter Feix
Article

Summary

An endosperm derived tissue culture of maize (Zea mays L.) variety A636 has been characterised for its ability to synthesize zein protein and respond to a zein gene regulatory element. Western analysis with zein specific antibodies revealed the distinct presence of zein proteins of the 15, 19 and 21 kDa classes in this tissue, in contrast to an embryo-derived Black Mexican Sweet variety tissue culture which exhibited no zein proteins. Transient transformation studies with a cauliflower mosaic virus 35S promoter and luciferase reporter gene construct demonstrate that protoplasts from this tissue culture, but not from the embryo-derived culture, respond positively to the potential enhancer which is located approximately 300 base pairs upstream of the coding region in most zein genes of maize, thus establishing the usefulness of this culture for studies of tissue specific gene regulation.

Key words

Maize Endosperm Tissue culture Regulation Transformation 

Abbreviations

MS

Murashige and Skoog

CaMV

cauliflower mosaic virus

BMS

Black Mexican Sweet

2,4-D

2,4-dichlorophenoxy — acetic acid

DAP

days after pollination

SDS

sodium dodecyl sulfate

PAGE

polyacrylamide gel electrophoresis

MES

2-morpholinoethane-sulfonic acid

Hepes

N-2-hydroxyethylpiper-azine-N′-2-ethansulfonic acid

Tris

Tris-(hydroxymethyl)-aminomethane

EDTA

ethyl-enediaminetetra-acetic acid disodium salt

PEG

polyethylene glycol

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Copyright information

© Springer-Verlag 1991

Authors and Affiliations

  • Tom J. A. Quayle
    • 1
  • Winfried Hetz
    • 1
  • Günter Feix
    • 1
  1. 1.Institut für Biologie III, Universität FreiburgFreiburgFRG

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