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Plant Cell Reports

, Volume 9, Issue 8, pp 415–418 | Cite as

Silicon carbide fiber-mediated DNA delivery into plant cells

  • Heidi F. Kaeppler
  • Weining Gu
  • David A. Somers
  • Howard W. Rines
  • Andrew F. Cockburn
Article

Summary

Silicon carbide fiber-mediated delivery of DNA into intact plant cells was investigated. Black Mexican Sweet (BMS) maize (Zea mays) and tobacco (Nicotiana tabacum) suspension culture cells were vortexed in the presence of liquid medium, plasmid DNA encoding β-glucuronidase (GUS), and silicon carbide fibers. Penetration of BMS cells by the silicon carbide fibers was observed by scanning electron microscopy of vortexed cells. Following fiber and DNA treatment, BMS cells transiently expressed GUS activity at a mean frequency of 139.5 units (one unit = one blue cell or one colony of blue cells) per sample. Treated tobacco cells expressed an average of 373 GUS units per sample. Untreated controls did not exhibit GUS activity. These results indicate that the silicon carbide fibers-vortex procedure can be used to rapidly and inexpensively deliver foreign DNA into intact plant cells for investigations of transient gene expression.

Keywords

Carbide Silicon Carbide Suspension Culture Cell Nicotiana Tabacum Tobacco Cell 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Abbreviations

BMS

Black Mexican Sweet maize suspension cultures

MS

Murashige and Skoog salts

GUS

β-glucuronidase

2,4-D

2,4 dichlorophenoxyacetic acid

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Copyright information

© Springer-Verlag 1990

Authors and Affiliations

  • Heidi F. Kaeppler
    • 1
  • Weining Gu
    • 1
  • David A. Somers
    • 1
  • Howard W. Rines
    • 2
  • Andrew F. Cockburn
    • 3
  1. 1.Department of Agronomy and Plant GeneticsUniversity of MinnesotaSt. PaulUSA
  2. 2.USDA-Agricultural Research ServicePlant Science Research UnitSt. PaulUSA
  3. 3.USDA-Agricultural Research ServiceSouth Atlantic Area Insects Affecting Man and Animals Research LaboratoryGainesvilleUSA

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