Plant Cell Reports

, Volume 9, Issue 8, pp 415–418 | Cite as

Silicon carbide fiber-mediated DNA delivery into plant cells

  • Heidi F. Kaeppler
  • Weining Gu
  • David A. Somers
  • Howard W. Rines
  • Andrew F. Cockburn


Silicon carbide fiber-mediated delivery of DNA into intact plant cells was investigated. Black Mexican Sweet (BMS) maize (Zea mays) and tobacco (Nicotiana tabacum) suspension culture cells were vortexed in the presence of liquid medium, plasmid DNA encoding β-glucuronidase (GUS), and silicon carbide fibers. Penetration of BMS cells by the silicon carbide fibers was observed by scanning electron microscopy of vortexed cells. Following fiber and DNA treatment, BMS cells transiently expressed GUS activity at a mean frequency of 139.5 units (one unit = one blue cell or one colony of blue cells) per sample. Treated tobacco cells expressed an average of 373 GUS units per sample. Untreated controls did not exhibit GUS activity. These results indicate that the silicon carbide fibers-vortex procedure can be used to rapidly and inexpensively deliver foreign DNA into intact plant cells for investigations of transient gene expression.


Carbide Silicon Carbide Suspension Culture Cell Nicotiana Tabacum Tobacco Cell 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.



Black Mexican Sweet maize suspension cultures


Murashige and Skoog salts




2,4 dichlorophenoxyacetic acid


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. Armstrong CL, Green CE (1985) Establishment and maintenance of friable, embryogenic maize callus and the involvement of L-proline. Planta 164:207–214Google Scholar
  2. Chu CC, Wang CC, Sun SC, Hsu C, Yin KC, Chu CY, Bi FY (1975) Establishment of an efficient medium for anther culture of rice through comparative experiments on the nitrogen sources. Sci Sin (Chin Ed) 18:658–659Google Scholar
  3. Crossway A, Oakes JV, Irvine JM, Ward B, Knauf VC, Shewmaker CK (1986) Integration of foreign DNA following microinjection of tobacco mesophyll protoplasts. Mol Gen Genet 202:179–185Google Scholar
  4. Finer JJ, McMullen MD (1990) Transformation of cotton (Gossypium hirsutum L.) via particle bombardment. Plant Cell Rep 8:586–589Google Scholar
  5. Gamborg OL, Miller RA, Ojima K (1968) Nutrient requirements of suspension cultures of soybean root cells. Exp. Cell Res. 50:151–158.Google Scholar
  6. Green CE (1977) Prospects for crop improvement in the field of cell culture. HortScience 12:131–134Google Scholar
  7. Jefferson RA (1987) Assaying chimeric genes in plants: the GUS gene fusion system. Plant Molec Biol Rep 5:397–405Google Scholar
  8. Klein TM, Wolf ED, Wu R, Sanford JC (1987) High velocity microprojectiles for delivery of nucleic acids into living cells. Nature 327:70–73Google Scholar
  9. Klein TM, Harper EC, Svab Z, Sanford JC, Fromm ME, Maliga P (1988) Stable genetic transformation of intact Nicotiana cells by the particle bombardment process. Proc Nat Acad Sci (USA) 85:8502–8505Google Scholar
  10. Klein TM, Kornstein L, Sanford JC, Fromm ME (1989) Genetic transformation of maize cells by particle bombardment. Plant Physiol 91:440–444Google Scholar
  11. McCabe DE, Swain WF, Martinell BJ, Christou P (1988) Stable transformation of soybean (Glycine max) by particle acceleration. Bio/Technology 6:923–926Google Scholar
  12. Murashige T, Skoog F (1962) A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol Plant 15:473–497Google Scholar
  13. Smith JA, Green CE, Gengenbach BG (1984) Feeder layer support of low density populations of Zea mays L. suspension cells. Plant Sci Lett 36:67–72Google Scholar
  14. Wang YC, Klein TM, Fromm M, Cao J, Sanford JC, Wu R. (1988) Transient expression of foreign genes in rice, wheat, and soybean cells following particle bombardment. Plant Mol Biol 11:433–439Google Scholar

Copyright information

© Springer-Verlag 1990

Authors and Affiliations

  • Heidi F. Kaeppler
    • 1
  • Weining Gu
    • 1
  • David A. Somers
    • 1
  • Howard W. Rines
    • 2
  • Andrew F. Cockburn
    • 3
  1. 1.Department of Agronomy and Plant GeneticsUniversity of MinnesotaSt. PaulUSA
  2. 2.USDA-Agricultural Research ServicePlant Science Research UnitSt. PaulUSA
  3. 3.USDA-Agricultural Research ServiceSouth Atlantic Area Insects Affecting Man and Animals Research LaboratoryGainesvilleUSA

Personalised recommendations