Plant Cell Reports

, Volume 9, Issue 4, pp 216–220 | Cite as

Efficient plant regeneration from rice protoplasts in general medium

  • Zhijian Li
  • Norimoto Murai
Article
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Revised:

Summary

We established an efficient and reproducible procedure for protoplast propagation and fertile plant regeneration of rice (Oryza sativa L.) cultivars Nipponbare and Taipei 309. Selection of scutellum-derived secondary calli, the use of General medium and nurse culture were all found to be critical in the procedure. When 5 basal media (Murashige and Skoog, RY-2, modified R2, Amino Acid and General media) were compared, suspension callus growth rate, protoplast yield and plating efficiency were all about 30% higher in General medium than in the second-best R2 medium. Only one month was required to develop suspension cultures for protoplast isolation using General medium. A plating efficiency as high as 17% and a plant regeneration frequency of 67% were achieved by the improved procedure. Agronomic traits of protoplast- and seed-derived plants were found to be similar.

Keywords

Plant Regeneration Agronomic Trait Oryza Sativa General Medium Callus Growth 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Abbreviations

AA

Amino Acid medium (Muller and Grafe 1978)

MS

Murashige and Skoog (1962) medium

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Copyright information

© Springer-Verlag 1990

Authors and Affiliations

  • Zhijian Li
    • 1
  • Norimoto Murai
    • 1
  1. 1.Department of Plant Pathology and Crop PhysiologyCollege of Agriculture, Louisiana State UniversityBaton RougeUSA

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