Abstract
Induction of single and multiple shoots was obtained from nodal expiants of 60–80 year-old elite trees of rosewood on Murashige and Skoog's basal medium supplemented with 6-benzylaminopurine (1.0 mg 1-1) and δ -Naphthalene acetic acid (0.05 mg 1-1) or indole acetic acid (0.5 mg 1-1). Multiplication of shoots was obtained on MS (reduced major elements) or Woody Plant Medium supplemented with 6-benzylaminopurine (1.0 mg 1-1) and kinetin (0.5–1.0 mg 1-1). Excised shoots were rooted on half-strength MS with IBA (2.0 mg 1-1) to obtain complete plantlets. The regenerated plantlets have been acclimatized and successfully transferred to the soil.
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Abbreviations
- MS:
-
Murashige and Skoog's (1962) medium
- B5:
-
Gamborg (1968) medium
- WPM:
-
Woody plant medium, Lloyd and McCown (1981) medium
- NAA:
-
δ-naphthalene acetic acid
- IAA:
-
indole-3-acetic acid
- IBA:
-
indole-3-butyric acid
- BAP:
-
6-benzyl aminopurine
- KIN:
-
kinetin
- PVP:
-
polyvinyl pyrrolidone
- CH:
-
casein hydrolysate
- ADS:
-
adenine sulphate
- L-Gl:
-
L-glutamine
- L-Arg:
-
L-arginine
- L-Asp:
-
L-asparagine
- PG:
-
phloroglucinol
References
Abo-el-Nil MM (1987) In: Bonga 3M, Durzan D (eds) Cell and Tissue Culture in Forestry, Vol 3, Martinus Nijhoff, Dordrecht, pp 400–410.
Anderson WC (1975) Proc Int Plant Prop Soc 25: 129–135.
Blake J (1978) In: In vitro multiplication of woody species, Round-Table Conference, Centre de Recherches Agronomiques de l'Etat, CRA, Gembloux, Belgium, pp 49.
Bonga JM (1977) In: Reinert J, Bajaj YPS (eds) Application of tissue culture in Forestry, Springer-Verlag, Berlin, pp 93–108.
Cohen D, Elliott D (1979) Proc Int Plant Prop Soc 26: 330–333.
Dhawan V, Bhojwani SS (1985) Plant Cell Reports 4: 315–318.
Gamborg OL, Miller RA, Ojima K (1968) Exp Cell Res 50: 151–158.
Gamborg OL, Murashige T, Thrope A, Vasil IK (1976) In Vitro 12: 473–478.
Gupta PK, Mascarenhas AF (1987) In: Bonga JM, Durzan D (eds) Cell and Tissue Culture in Forestry, Vol 3, Martinus Nijhoff, Dordrecht, pp 385–389.
Jones OP (1976) Nature 262: 392–393.
Lakshmi Sita G, Chattopadhyay S, Tejavathi DH, (1986) Plant Cell Reports 5: 266–268.
Lloyd G, McCown BH (1981) Proc Int Plant Prop Soc 30: 421–427.
McComb JA (1978) Proc Int Plant Prop Soc 28: 413–426.
McComb JA, Bennett, U In: Fujiwara A (ed) Plant Tissue Culture 1982, Maruzen, Tokyo, pp 721–722.
McCown BH, Amos R (1979) Proc Int Plant Prop Soc 29: 387–393.
Murashige T, Skoog F (1962) Physiol Plant 15: 473–497.
Nagmani R, Venkateswaran S (1987) In: Bonga JM, Durzan D (eds) Cell and Tissue Culture in Forestry, Vol 3, Martinus Nijhoff, Dordrecht, pp 285–291.
Nataraja K, Sudhadevi AM (1984) Beitr Biol Pflanz 59: 341–349.
NRC (1979) In: Tropical Legumes: Resources for the future, Luxury Timbers: Rosewoods, National Academy of Sciences, Washington DC, USA, pp 231–238.
Raghava Swamy BV, Lakshmi Sita G (1990) In: Proceedings of the Abstracts of the International Seminar on New Frontiers in Horticulture, Indo-American Hybrid Seeds, Bangalore, India, pp 147.
Ravishankar Rai V, Jagadish Chandra KS (1989) Ann Bot 64: 43–46.
Sha L, McCown BH, Peterson L (1985) J Amer Soc Hortic Sci 110: 631–634.
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Communicated by J. K. Vasil
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Swamy, B.V.R., Himabindu, K. & Sita, G.L. In vitro micropropagation of elite rosewood (Dalbergia latifolia Roxb.). Plant Cell Reports 11, 126–131 (1992). https://doi.org/10.1007/BF00232164
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DOI: https://doi.org/10.1007/BF00232164