Advertisement

Plant Cell Reports

, Volume 11, Issue 3, pp 126–131 | Cite as

In vitro micropropagation of elite rosewood (Dalbergia latifolia Roxb.)

  • B. V. Raghava Swamy
  • K. Himabindu
  • G. Lakshmi Sita
Article

Abstract

Induction of single and multiple shoots was obtained from nodal expiants of 60–80 year-old elite trees of rosewood on Murashige and Skoog's basal medium supplemented with 6-benzylaminopurine (1.0 mg 1-1) and δ -Naphthalene acetic acid (0.05 mg 1-1) or indole acetic acid (0.5 mg 1-1). Multiplication of shoots was obtained on MS (reduced major elements) or Woody Plant Medium supplemented with 6-benzylaminopurine (1.0 mg 1-1) and kinetin (0.5–1.0 mg 1-1). Excised shoots were rooted on half-strength MS with IBA (2.0 mg 1-1) to obtain complete plantlets. The regenerated plantlets have been acclimatized and successfully transferred to the soil.

Keywords

Acetic Acetic Acid Naphthalene Indole Basal Medium 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Abbreviations

MS

Murashige and Skoog's (1962) medium

B5

Gamborg (1968) medium

WPM

Woody plant medium, Lloyd and McCown (1981) medium

NAA

δ-naphthalene acetic acid

IAA

indole-3-acetic acid

IBA

indole-3-butyric acid

BAP

6-benzyl aminopurine

KIN

kinetin

PVP

polyvinyl pyrrolidone

CH

casein hydrolysate

ADS

adenine sulphate

L-Gl

L-glutamine

L-Arg

L-arginine

L-Asp

L-asparagine

PG

phloroglucinol

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. Abo-el-Nil MM (1987) In: Bonga 3M, Durzan D (eds) Cell and Tissue Culture in Forestry, Vol 3, Martinus Nijhoff, Dordrecht, pp 400–410.Google Scholar
  2. Anderson WC (1975) Proc Int Plant Prop Soc 25: 129–135.Google Scholar
  3. Blake J (1978) In: In vitro multiplication of woody species, Round-Table Conference, Centre de Recherches Agronomiques de l'Etat, CRA, Gembloux, Belgium, pp 49.Google Scholar
  4. Bonga JM (1977) In: Reinert J, Bajaj YPS (eds) Application of tissue culture in Forestry, Springer-Verlag, Berlin, pp 93–108.Google Scholar
  5. Cohen D, Elliott D (1979) Proc Int Plant Prop Soc 26: 330–333.Google Scholar
  6. Dhawan V, Bhojwani SS (1985) Plant Cell Reports 4: 315–318.Google Scholar
  7. Gamborg OL, Miller RA, Ojima K (1968) Exp Cell Res 50: 151–158.PubMedGoogle Scholar
  8. Gamborg OL, Murashige T, Thrope A, Vasil IK (1976) In Vitro 12: 473–478.Google Scholar
  9. Gupta PK, Mascarenhas AF (1987) In: Bonga JM, Durzan D (eds) Cell and Tissue Culture in Forestry, Vol 3, Martinus Nijhoff, Dordrecht, pp 385–389.Google Scholar
  10. Jones OP (1976) Nature 262: 392–393.Google Scholar
  11. Lakshmi Sita G, Chattopadhyay S, Tejavathi DH, (1986) Plant Cell Reports 5: 266–268.Google Scholar
  12. Lloyd G, McCown BH (1981) Proc Int Plant Prop Soc 30: 421–427.Google Scholar
  13. McComb JA (1978) Proc Int Plant Prop Soc 28: 413–426.Google Scholar
  14. McComb JA, Bennett, U In: Fujiwara A (ed) Plant Tissue Culture 1982, Maruzen, Tokyo, pp 721–722.Google Scholar
  15. McCown BH, Amos R (1979) Proc Int Plant Prop Soc 29: 387–393.Google Scholar
  16. Murashige T, Skoog F (1962) Physiol Plant 15: 473–497.Google Scholar
  17. Nagmani R, Venkateswaran S (1987) In: Bonga JM, Durzan D (eds) Cell and Tissue Culture in Forestry, Vol 3, Martinus Nijhoff, Dordrecht, pp 285–291.Google Scholar
  18. Nataraja K, Sudhadevi AM (1984) Beitr Biol Pflanz 59: 341–349.Google Scholar
  19. NRC (1979) In: Tropical Legumes: Resources for the future, Luxury Timbers: Rosewoods, National Academy of Sciences, Washington DC, USA, pp 231–238.Google Scholar
  20. Raghava Swamy BV, Lakshmi Sita G (1990) In: Proceedings of the Abstracts of the International Seminar on New Frontiers in Horticulture, Indo-American Hybrid Seeds, Bangalore, India, pp 147.Google Scholar
  21. Ravishankar Rai V, Jagadish Chandra KS (1989) Ann Bot 64: 43–46.PubMedGoogle Scholar
  22. Sha L, McCown BH, Peterson L (1985) J Amer Soc Hortic Sci 110: 631–634.Google Scholar

Copyright information

© Springer-Verlag 1992

Authors and Affiliations

  • B. V. Raghava Swamy
    • 1
  • K. Himabindu
    • 1
  • G. Lakshmi Sita
    • 1
  1. 1.Plant Tissue Culture Laboratory, Department of Microbiology and Cell BiologyIndian Institute of ScienceBangaloreIndia

Personalised recommendations