Plant Cell Reports

, Volume 11, Issue 3, pp 126–131 | Cite as

In vitro micropropagation of elite rosewood (Dalbergia latifolia Roxb.)

  • B. V. Raghava Swamy
  • K. Himabindu
  • G. Lakshmi Sita


Induction of single and multiple shoots was obtained from nodal expiants of 60–80 year-old elite trees of rosewood on Murashige and Skoog's basal medium supplemented with 6-benzylaminopurine (1.0 mg 1-1) and δ -Naphthalene acetic acid (0.05 mg 1-1) or indole acetic acid (0.5 mg 1-1). Multiplication of shoots was obtained on MS (reduced major elements) or Woody Plant Medium supplemented with 6-benzylaminopurine (1.0 mg 1-1) and kinetin (0.5–1.0 mg 1-1). Excised shoots were rooted on half-strength MS with IBA (2.0 mg 1-1) to obtain complete plantlets. The regenerated plantlets have been acclimatized and successfully transferred to the soil.


Acetic Acetic Acid Naphthalene Indole Basal Medium 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.



Murashige and Skoog's (1962) medium


Gamborg (1968) medium


Woody plant medium, Lloyd and McCown (1981) medium


δ-naphthalene acetic acid


indole-3-acetic acid


indole-3-butyric acid


6-benzyl aminopurine




polyvinyl pyrrolidone


casein hydrolysate


adenine sulphate










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Copyright information

© Springer-Verlag 1992

Authors and Affiliations

  • B. V. Raghava Swamy
    • 1
  • K. Himabindu
    • 1
  • G. Lakshmi Sita
    • 1
  1. 1.Plant Tissue Culture Laboratory, Department of Microbiology and Cell BiologyIndian Institute of ScienceBangaloreIndia

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