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Plant Cell Reports

, Volume 9, Issue 1, pp 47–50 | Cite as

Production of isolated somatic embryos from sunflower thin cell layers

  • Bernard Pélissier
  • Ouafa Bouchefra
  • Régis Pépin
  • Georges Freyssinet
Article

Abstract

We describe here a two step procedure which allows the easy isolation of somatic embryos from Sunflower (Helianthus annuus L.) hypocotyl tissues. Thin cell layers composed of the epidermis plus 3 to 6 parenchyma cell layers were incubated for 5 days in a basal Murashige and Skoog medium using an auxin to cytokinin weight ratio of 1/1. The epidermis layers were then transferred to a Gamborg medium containing a high level of sucrose. After one week of incubation in this medium, many somatic embryos started to be released from the parental epidermal tissue. Even though the germination of these embryos is difficult, we have been able to induce secondary embryos and regenerate fertile plants.

Keys words

sunflower thin cell layers somatic embryos 

Abbreviations

NAA

1-naphthalene acetic acid

IAA

indole-3-acetic acid

BAP

6-benzylamino-purine

MS

Murashige and Skoog medium

B5

Gamborg medium

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Copyright information

© Springer-Verlag 1990

Authors and Affiliations

  • Bernard Pélissier
    • 1
  • Ouafa Bouchefra
    • 1
  • Régis Pépin
    • 1
  • Georges Freyssinet
    • 1
  1. 1.Rhône-Poulenc Agrochimie, Biologie Moléculaire et Cellulaire VégétaleLyon Cédex 09France

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