Plant Cell Reports

, Volume 9, Issue 9, pp 484–487

Verbascoside production by plant cell cultures

  • Nobuyuki Inagaki
  • Hiroaki Nishimura
  • Minoru Okada
  • Hiroshi Mitsuhashi
Article

Abstract

Verbascoside was found to be produced in all calli derived from eleven species that contained the compound in their leaves. Cell suspension cultures were also established in three species, i.e., Leucosceptrum japonicum f. barbinerve, Syringa josikaea, and Sy. vulgaris, all of which were found to produce verbascoside at more than 1 g/l. Of the three species, suspension cultures of L. japonicum f. barbinerve showed rapid growth and the highest yield of verbascoside (1.89 g/l). In these cultures, the effects of major salt concentration in B5 medium on cell growth and verbascoside production were examined. Maximum cell growth and maximum verbascoside production were both achieved by reducing the major salt concentration to half that of the original medium.

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Copyright information

© Springer-Verlag 1991

Authors and Affiliations

  • Nobuyuki Inagaki
    • 1
  • Hiroaki Nishimura
    • 1
  • Minoru Okada
    • 1
  • Hiroshi Mitsuhashi
    • 1
  1. 1.Research Institute for Biology and Chemistry, Tsumura and Co.IbarakiJapan

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