Plant Cell Reports

, Volume 9, Issue 3, pp 147–150 | Cite as

Extracellular accumulation of high specific-activity peroxidase by cell suspension cultures of cowpea

  • Oscar A. V. Moreno
  • Rafael Vazquez-Duhalt
  • Hector Nolasco
Article

Abstract

Cell suspension cultures of cowpea (Vigna sp.) were able to produce extracellular peroxidase. Different growth regulator concentrations induced different peroxidase activity in callus. The crude extracellular medium after four weeks of culture showed higher (6 times) specific peroxidase activity and higher thermo stability than commercial horse-radish peroxidase. The commercial production of peroxidase enzyme from cowpea by utilizing plant cell cultures is discussed.

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. Alberti BN, Klivanov AM (1981) Biotechnol. Bioeng. Symp. 11:373–379.Google Scholar
  2. Bakardjieva NT, Irzvoska ND, Hristova N (1987) Comptes Rendus Acad. Bulg. Sci. 40(8):85–88.Google Scholar
  3. Bredemeijer GM, Burg HC, Sree-Ramalu K, Dijkhuis P (1985) Acta Bot. Neerl. 34:325–335.Google Scholar
  4. Castillo FJ, Penel C, Greppin H (1984) Plant Physiol. 74:846–851.Google Scholar
  5. Chibbar RN, Cella R, Albany D, van Huystee RB (1984) J. Exp. Bot. 35:1846–1852.Google Scholar
  6. Cuenca J, Garcia-Florenciano E, Ros Barcelo A, Munoz R (1989) Plant Cell Reports8:471–474.Google Scholar
  7. Druart P, Kevers C, Boxus P, Gaspar T (1982) Z. Pflanzenphysiol. 108:429–436.Google Scholar
  8. Fils B, Sauvage FX, Nicolas J (1985) Sciences des Aliments 5:217–232.Google Scholar
  9. Fry SC (1980) Phytochemestry 19:735–740.Google Scholar
  10. Gaspar Th, Penel C, Thorpe T, Greppin H (1982) Peroxidases 1970–1980. A survey of their Biochemical and Phisiological Roles in Higher Plants. Universite de Geneve, Centre de Botanique, Geneve, Switzeland.Google Scholar
  11. Gaspar Th, Penel C, Castillo F, Greppin H (1985) Physiol. Plantarum 64:418–423.Google Scholar
  12. Kermasha S, Metche M (1988) J. Food Sci. 53 (1):247–252.Google Scholar
  13. Klivanov AM, Alberti BM, Morris ED, Felshin LM (1980) J. Appl. Biochem. 2:414–421.Google Scholar
  14. Laemmli UK (1970) Nature 227:680–685.PubMedGoogle Scholar
  15. Lowry OH, Rosebrough NJ, Lewis-Farr AL, Randall R (1951) J. Biol. Chem. 193:265–275.PubMedGoogle Scholar
  16. Mato MC, Rua ML, Ferro E (1988) Physiol. Plantarum 72:84–88.Google Scholar
  17. Moncousin C (1986) In Molecular and Physiological aspects of plant peroxidases (Greppin H, Penel C, Gaspar Th editors) pp 379–385 Universite de Geneve, Centre de Botanique.Google Scholar
  18. Moncousin C, Gaspar Th (1983) Biochem. Physiol. Pflanz. 178:263–271.Google Scholar
  19. Mellon JE (1986) Plant Cell Reports 5:338–341.Google Scholar
  20. Moreno OA, Vazquez-Duhalt R, Ochoa JL (1989) Plant Cell Tissue and Organ Culture 18:321–327.Google Scholar
  21. Murashige T, Skoog F (1962) Physiol. Plant 15:473–497.Google Scholar
  22. Sesto PA, van Huystee RB (1989) Plant Sci. 61:163–168.Google Scholar
  23. van Huystee RB (1987) Plant Peroxidase. In: Isoenzymes; Current Topics in Biological and Medical Research 16:241-245 Agric. Physiol. Med.Google Scholar
  24. van Huystee RB, Tam ASK (1988) J. Plant Physiol. 133:645–647.Google Scholar
  25. Yamada Y, Kobayashi S, Watanabe K, Hayashi U (1987) J. Chem. Tech. Biotechnol. 38:31–39.Google Scholar

Copyright information

© Springer-Verlag 1990

Authors and Affiliations

  • Oscar A. V. Moreno
    • 1
  • Rafael Vazquez-Duhalt
    • 1
  • Hector Nolasco
    • 1
  1. 1.Centro de Investigaciones Biológicas de Baja California SurLa Paz, Baja California SurMéxico

Personalised recommendations