Plant Cell Reports

, Volume 15, Issue 6, pp 427–430 | Cite as

Micropropagation of Hemidesmus indicus (L.) R. Br. through axillary bud culture

  • J. Patnaik
  • B. K. Debata
Article
Received:
Revised:

Abstract

A procedure for rapid in vitro propagation of the aromatic and medicinal plant Hemidesmus indicus (L.) R.Br. (Family Asclepiadaceae) from nodal explants is described. The highest shoot multiplication rate of 8.2 ± 0.4 shoots/explant with a 95% frequency was achieved in S weeks culture period on Murashige and Skoog medium supplemented with 1.15 μM kinetin and 0.054 μM α-naphthaleneacetic acid. Excised shoots were rooted on the same basal medium supplemented with 1.15 μM kinetin and 7.35 μM indole-3-butyric acid. Shoots derived from subcultures exhibited better rooting response than those from primary cultures. After a hardening phase of 2 weeks, there was a 70% transplantation success in the field.

Keywords

Medicinal Plant Primary Culture Multiplication Rate Kinetin Culture Period 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Abbreviations

MS

Murashige and Skoog (1962) medium

BA

N6 benzyladenine; KN kinetin

NAA

a-naphthaleneacetic acid

IBA

indole-3-butyric acid

IAA

indole-3-acetic acid

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Copyright information

© Springer-Verlag 1996

Authors and Affiliations

  • J. Patnaik
    • 1
  • B. K. Debata
    • 1
  1. 1.Aromatic and Medicinal Plants DivisionRegional Research LaboratoryBhubaneswarIndia

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