Plant Cell Reports

, Volume 14, Issue 9, pp 589–592 | Cite as

Transformation of grape (Vitis vinifera L.) zygotic-derived somatic embryos and regeneration of transgenic plants

  • R. Scorza
  • J. M. Cordts
  • D. W. Ramming
  • R. L. Emershad


Transgenic grape plants were regenerated from somatic embryos derived from immature zygotic embryos of seedless grape (Vitis vinifera L.) selections. Somatic embryos were bombarded twice with 1 μm gold particles using the Biolistic PDS-1000/He device (Bio-Rad Laboratories) and then exposed to Agrobacterium tumefaciens strain C58/Z707 containing the binary plasmid pGA482GG or pCGN7314. Following cocultivation, secondary embryos were allowed to proliferate on Emershad/Ramming proliferation (ERP) medium for 6 weeks before selection on ERP medium containing 20–40 μg/ml kanamycin (kan). Transgenic embryos were identified after 3–5 months under selection and allowed to germinate and develop into rooted plants on Woody Plant Medium containing 1 μM 6-benzylaminopurine (BAP), 1.5% sucrose, 0.3% activated charcoal and 0.75% agar. Integration of the foreign genes into these grapevines was verified by growth in the presence of kan, positive GUS and PCR assays, and Southern analysis.


Charcoal Somatic Embryo Activate Charcoal Zygotic Embryo Vitis Vinifera 
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  1. Baribault TJ, Skene KGM, Scott NS (1989) Plant Cell Reports 8:137–140Google Scholar
  2. Baribault TJ, Skene KGM, Cain PA, Scott NS (1990) J Expt Bot 41:1045–1049Google Scholar
  3. Berres R, Otten L, Tinland B, Malgarinin-Clog E, Walter B (1992) Plant Cell Rep 11:192–195Google Scholar
  4. Bidney D, Scelonge C, Martich J, Burrus M, Sims L, Huffman G (1992) Plant Mol Biol 18:301–313Google Scholar
  5. California Table Grape Commission (1993) 1992–1993: The distribution and per capita consumption of California table grapes by major varieties in the United States and Canada. PO Box 5498, Fresno, CA 93755Google Scholar
  6. Callahan AM, Morgens PH, Wright P, Nichols Jr KE (1992) Plant Physiol 100:482–488.Google Scholar
  7. Chee PP, Fober KA, Slightom JL (1989) Plant Physiol 91:1212–1218Google Scholar
  8. Clog E, Bass P, Walter B (1990) Plant Cell Rep 8:726–728Google Scholar
  9. Colby SM, Juncosa AM, Meredith CP (1991) J Amer Soc Hort Sci 116:356–361Google Scholar
  10. Comai L, Moran P, Maslyar D (1990) Plant Mol Biol 15:373–381Google Scholar
  11. Emershad RL, Ramming DW (1994) Plant Cell Rep 14:6–12Google Scholar
  12. Guellec V, David C, Branchard M, Tempe J (1990) Plant Cell Tiss Org Cult 20:211–215Google Scholar
  13. Hébert D, Kikkert JR, Smith FD, Reisch BI (1993) Plant Cell Rep 12:585–589Google Scholar
  14. Jefferson RA (1987) Plant Mol Biol Rep 5:387–405Google Scholar
  15. Lloyd G, McCown BH (1981) Proc Int Plant Prop Soc 30:421–427Google Scholar
  16. Margosan DA, Emershad RL, Ramming DW (1994) Inter Symp Table Grape Production, Anaheim, Ca, USA p 133–135Google Scholar
  17. Mullins MG, Tang FCA, Facciotti D (1990) Bio/Technology 8:1041–1045Google Scholar
  18. Murashige T, Skoog G (1962) Physiol Plant 15:473–497Google Scholar
  19. Nakano M, Hoshino Y, Mii M (1994) J Expt Bot 45:649–656Google Scholar
  20. Norstog K (1973) In Vitro 8:307–308Google Scholar
  21. Sanford JC, Smith FD, Russell JA (1991) Meth Enzymol 217:483–509Google Scholar
  22. Scorza R, Morgens PH, Cordts JM, Mante S, Callahan AM (1990) In Vitro Cell Devel Biol 26:829–834Google Scholar
  23. Scorza R, Zimmerman TW, Cordts JM, Footen KJ, Ravelonandro M (1994) J Amer Soc Hort Sci 119:1091–1098Google Scholar
  24. Slightom JL (1991) Gene 100:252–255Google Scholar
  25. Slightom JL, Drong RF, Sieu LC, Chee PP (1991) In: SB Gelvin, RA Schilperoot and DPS Venna (Eds), Plant Molec Biol Man B 16, pp. 1–55, Kluwer Academic Pub, Dordrecht, The NetherlandsGoogle Scholar
  26. Stamp JA, Colby SM, Meredith CP (1990) J Amer Soc Hort Sci 115:1038–1042Google Scholar
  27. While PR (1954) The cultivation of animal and plant cells. Ronald Press, NYGoogle Scholar

Copyright information

© Springer-Verlag 1995

Authors and Affiliations

  • R. Scorza
    • 1
  • J. M. Cordts
    • 1
  • D. W. Ramming
    • 2
  • R. L. Emershad
    • 2
  1. 1.USDA-ARS Appalachian Fruit Research StationKearneysvilleUSA

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