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Sexual Plant Reproduction

, Volume 6, Issue 4, pp 244–248 | Cite as

Involvement of phenylalanine ammonia-lyase in the development of pollen in broccoli (Brassica oleracea L.)

  • S. Kishitani
  • A. Yomoda
  • N. Konno
  • Y. Tanaka
Original Articles

Summary

To determine whether phenylalanine ammonia-lyase (EC 4.3.1.5) is involved in the maturation of microspores to fertile pollen, anthers of a fertile strain of broccoli (Brassica oleracea L.) were studied in a comparison with anthers of a cytoplasmic male sterile strain. In the normal fertile strain, immature anthers of about 2 mm in length exhibited higher phenylalanine ammonia-lyase activity than mature anthers or those shorter than 2 mm. The 2-mm-long anthers corresponded to the mononucleate stage, just after release of the microspores during pollen development. Immunohistochemical localization of phenylalanine ammonia-lyase in the anthers indicated that the protein was present predominantly in the tapetal cells. The immature anthers of cytoplasmic male sterile broccoli had a lower phenylalanine ammonia-lyase activity than those of the normal fertile strain. The level of phenylalanine ammonia-lyase activity in the immature anthers was positively correlated with the number of fertile pollen grains at the flowering stage in both strains. It seems possible, therefore, that phenylpropanoid metabolism, which involves phenylalanine ammonia-lyase, may play an important role in the maturation of microspores in flowering plants.

Key words

Brassica oleracea L. Broccoli Cytoplasmic male sterility Phenylalanine ammonia-lyase Pollen development 

Abbreviations

CHS

chalcone synthase

CMS

cytoplasmic male sterility

DAPI

4′, 6-diamidmo-2-phenylindole dihydrochloride

PAL

L-phenylalanine ammonia-lyase

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Copyright information

© Springer-Verlag 1993

Authors and Affiliations

  • S. Kishitani
    • 1
  • A. Yomoda
    • 1
  • N. Konno
    • 1
  • Y. Tanaka
    • 2
  1. 1.Laboratory of Plant Breeding, Faculty of AgricultureTohoku UniversitySendaiJapan
  2. 2.National Institute of Agro-biological ResearchTsukubaJapan

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