Ethyl m-aminobenzoate methanesulfonate dependent and carrier dependent pharmacokinetics of extremely lipophilic compounds in rainbow trout

  • Dick T. H. M. Sijm
  • Jaap Bol
  • Willem Seinen
  • Antoon Opperhuizen


Significant differences were found in both uptake and pharmacokinetics in fish when six lipophilic compounds were dosed by gavage in either an oil or an gelatin carrier. Pharmacokinetics were also different when fish were anaesthetized with ethyl m-aminobenzoate methanesulfonate (MS-222) before dosing. The highest uptake percentages, uptake rates and concentrations of the compounds were found in the fish which were given the gelatin carrier only. MS-222 decreased the uptake of the compounds. Absorption of the compounds from oil was lower than from gelatin. In addition, absorption from oil continued for 21 d, which lasted longer than from gelatin. In the fish which were given the gelatin, the concentrations of the compounds in both muscle and liver showed a compound dependent decrease in the course of the experiment. In general, in all treatments higher concentrations were found in liver than in muscle. For each treatment, the absorption rates of pentachlorobenzene, hexachlorobenzene, 2,2′,5,5′-tetrachlorobiphenyl and 2,2′,4,4′,5,5′-hexachlorobiphenyl were similar, but different from the other treatments. It can be concluded that the experimental methodology significantly affects the pharmacokinetic parameters that are used to model the bioconcentration of hydrophobic chemicals in the environment.


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Copyright information

© Springer-Verlag 1993

Authors and Affiliations

  • Dick T. H. M. Sijm
    • 1
  • Jaap Bol
    • 1
  • Willem Seinen
    • 1
  • Antoon Opperhuizen
    • 1
  1. 1.Research Institute of Toxicology, Environmental Chemistry GroupUniversity of UtrechtUtrechtThe Netherlands

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