Human Genetics

, Volume 91, Issue 1, pp 31–36

Localization of a gamma-glutamyl-transferase-related gene family on chromosome 22

  • Christine Morris
  • Céline Courtay
  • Ad Geurts van Kessel
  • Johanna ten Hoeve
  • Nora Heisterkamp
  • John Groffen
Original Investigations

DOI: 10.1007/BF00230218

Cite this article as:
Morris, C., Courtay, C., van Kessel, A.G. et al. Hum Genet (1993) 91: 31. doi:10.1007/BF00230218

Abstract

A gene family encompassing a minimum of four genes or pseudogenes for gamma-glutamyl transferase (GGT; EC 2.3.2.2) is present on chromosome 22q11. We have previously isolated a cDNA related to GGT but clearly not belonging to its gene family. The chromosomal location of this related gene, GGTLA1, has been determined by both isotopic and fluorescence in situ hybridization to metaphase cells and by Southern blot analysis of somatic cell hybrid DNAs. We show that GGTLA1 is part of a distinct gene family, which has at least four members (GGTLA1, GGTLA2, GGTLA3, GGTLA4). At least two loci are located on chromosome 22 within band q11 and proximal to the chronic myelogenous leukemia (CML) breakpoint in BCR (breakpoint cluster region gene). At least one other member is located more distally between the breakpoints found in Ewings sarcoma and CML. Some of the GGT and GGTLA family members are located on NotI restriction enzyme fragments of a similar size. Combined results indicate that a segment of human chromosome 22q11 has undergone largescale amplification events relatively recently in evolution.

Copyright information

© Springer-Verlag 1993

Authors and Affiliations

  • Christine Morris
    • 1
  • Céline Courtay
    • 2
    • 4
  • Ad Geurts van Kessel
    • 3
  • Johanna ten Hoeve
    • 4
  • Nora Heisterkamp
    • 4
  • John Groffen
    • 4
  1. 1.Cytogenetic and Molecular Oncology UnitChristchurch HospitalChristchurchNew Zealand
  2. 2.Unité Associée 597Centre National de la Recherche ScientifiqueNancyFrance
  3. 3.Department of Human GeneticsUniversity Hospital NijmegenNijmegenThe Netherlands
  4. 4.Section of Molecular Diagnosis, Department of PathologyChildren's Hospital Los AngelesLos AngelesUSA

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