A novel stimulator of protein phosphorylation in Neurospora crassa
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An endogenous thermostable activator of Protein kinase III (PKIII) was purified from 100000 × g supernatants of Neurospora crassa mycelial extracts. This 38 000 dalton polypeptide, clearly separable from calmodulin on P-60 gel filtration, specifically stimulated N. crassa PKIII activity on casein or phosvitin “in vitro” phosphorylation.
The factor was only present in the initial growth phase of the fungus. The mechanism of PKIII activation and its possible regulatory role are discussed.
Key wordsprotein kinase regulation Neurospora crassa
2-N-Morpholino ethane-sulfonic acid
100000 × g Supernatant
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- 1.Nimmo HG, Cohen P: Hormonal control of protein phosphorylation. Adv Cycl Nuc Res 8:145–251, 1977Google Scholar
- 2.Krebs EG, Beavo JA: Phosphorylation-dephosphorylation of enzymes. Ann Rev Biochem 48:923–959, 1979Google Scholar
- 3.Krebs EG: Historical perspectives on protein phosphorylation and a classification system for protein kinaes -Phil-Trans-R. R Soc London 302:3–1, 1983Google Scholar
- 4.Judewicz ND, Glikin GC, Torres HN: Protein kinase activities in Neurospora crassa. Arch Biochem Biophys 206:87–92, 1981Google Scholar
- 5.Glikin GC, Judewicz ND, Torres HN: Protein kinase activities in Neurospora crassa- Molecular Properties. Mol Cell Biochem 46:121–126, 1982Google Scholar
- 6.Powers PA, Pall ML: Cyclic AMP-dependent protein kinase of Neurospora crassa. Biochem Biophys Res Comm 95:701–706, 1980Google Scholar
- 8.Glynn IM, Chappell JB: A simple method for the preparation of (32P)-labelled adenosine triphosphate of high specific activity. Biochem J 90:147–149, 1964Google Scholar
- 9.Téllez-Iñón MT, Ulloa RM, Glikin GC, Torres HN: Characterization of Neurospora crassa cyclic AMP phosphodiesterase activated by calmodulin. Biochem J 232:425–430, 1985Google Scholar