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Molecular and Cellular Biochemistry

, Volume 77, Issue 1, pp 11–17 | Cite as

A novel stimulator of protein phosphorylation in Neurospora crassa

  • Rita M. Ulloa
  • Gerardo C. Glikin
  • María T. Téllez-Ĩnón
  • Héctor N. Torres
  • Norberto D. Judewicz
Original Article

Summary

An endogenous thermostable activator of Protein kinase III (PKIII) was purified from 100000 × g supernatants of Neurospora crassa mycelial extracts. This 38 000 dalton polypeptide, clearly separable from calmodulin on P-60 gel filtration, specifically stimulated N. crassa PKIII activity on casein or phosvitin “in vitro” phosphorylation.

The factor was only present in the initial growth phase of the fungus. The mechanism of PKIII activation and its possible regulatory role are discussed.

Key words

protein kinase regulation Neurospora crassa 

Abbreviations

PK

protein kinase

MES

2-N-Morpholino ethane-sulfonic acid

PMSF

phenylmethylsulfonyl fluoride

S100

100000 × g Supernatant

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Copyright information

© Martinus Nijhoff Publishers 1987

Authors and Affiliations

  • Rita M. Ulloa
    • 1
    • 2
  • Gerardo C. Glikin
    • 1
    • 2
  • María T. Téllez-Ĩnón
    • 1
    • 2
  • Héctor N. Torres
    • 1
    • 2
  • Norberto D. Judewicz
    • 1
    • 2
  1. 1.Instituto de Investigaciones en Ingeniería Genética y Biología Molecular (INGEBI) CONICETBuenos AiresArgentina
  2. 2.Facultad de Ciencias Exactas y NaturalesBuenos AiresArgentina

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