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Theoretical and Applied Genetics

, Volume 93, Issue 7, pp 1071–1077 | Cite as

Microsatellite DNA markers for rice chromosomes

  • H. Akagi
  • Y. Yokozeki
  • A. Inagaki
  • T. Fujimura
Article

Abstract

We found 369 complete microsatellites, of which (CGG/GCC)n was the most frequent, in 11 798 rice sequences in the database. Of these microsatellites, 35 out of 45 could be successfully converted into microsatellite DNA markers using sequence information in their flanking regions. Thus, the time and labor used to develop new microsatellite DNA markers could be saved by using these published sequences. Twenty eight polymorphic markers between Asominori (japonica) and IR24 (indica) have been correctly mapped on the rice genome and microsatellites appear to be randomly distributed in the rice chromosomes. Integration of these markers with the published microsatellite DNA markers showed that about 35% of the rice chromosomes were covered by the 56 microsatellite DNA markers. These microsatellites were hypervariable and were easily to assay by PCR; they were distributed to all chromosomes and therefore, one can easily select plants carrying desired chromosome regions using these microsatellite DNA markers. Thus, microsatellite maps should aid the development of new breeds of rice saving time, labor, and money.

Key words

Microsatellite (simple sequence repeats) Polymorphisms Rice Genetic linkage maps PCR 

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Copyright information

© Springer-Verlag 1996

Authors and Affiliations

  • H. Akagi
    • 1
  • Y. Yokozeki
    • 1
  • A. Inagaki
    • 1
  • T. Fujimura
    • 1
  1. 1.Plant Biotechnology Laboratory, Life Science Institute, Mitsui Toatsu Chemicals Inc.MobaraJapan

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