Theoretical and Applied Genetics

, Volume 81, Issue 3, pp 406–412 | Cite as

Gametic selection in anther culture of rice (Oryza sauva L.)

  • E. Guiderdoni


The segregation and recombination of heterozygous isozyme markers have been monitored in anther culture derivatives (i.e., six nonmorphogenic microspore-derived callus [NMC] populations and two anther culture plant [ACP] populations) and F2 plants generated from six F1 hybrids of rice, including five japonica upland/improved indica tropical hybrids. The alleles in excess at some loci displaying skewed segregations in the F2s were consistently overrepresented in the NMC populations. These alleles were also generally found to be overabundant in the two ACP populations except for certain loci that contrastingly segregated in a 1∶1 ratio. Additional distortions were found to be specific to AC derivatives indicating the existence of in vitro gametic selection. Overall, however, the gametic selection in the ACP materials was neutral with regard to the indica and japonica differentiation. Estimates of linkages between markers borne by chromosome 6 using AC-derivative data were consistent with those noted in the F2s and with current knowledge of the isozyme locus linkage map. Given the average neutrality of gametic selection and the consistency of linkage relationships in the ACPs, their further use as rice molecular mapping and gene tagging populations can be investigated with confidence.

Key words

Rice Japonica/indica Anther culture Isozyme markers segregation Linkage 


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  1. Alexander MP (1969) Differential staining of aborted and non-aborted pollen. Stain Technol 44:117–122PubMedGoogle Scholar
  2. Bui Dang Ha D, Pernès J (1982) Androgenesis in pearl millet; analysis of plants obtained from microspore culture. Z Pflanzenphysiol 108:317–327Google Scholar
  3. Chen CC (1977) In vitro development of plants from microspores of rice. In Vitro 13:484–489Google Scholar
  4. Chen CC, Chiu WL, Yu LJ, Ren SS, Yu WJ (1983) Genetic analysis of anther-derived plants of rice; independent assortment of unlinked genes. Can J Genet Cytol 25:18–24Google Scholar
  5. Chen Y (1986) Anther and pollen culture of rice. In: Hu H, Yang H (eds) Haploids of higher plants in vitro. China Academic Publ, Beijing and Springer, Berlin Heidelberg New York, pp 3–25Google Scholar
  6. Chu CC (1978) The N6 medium and its application to anther culture of cereal crops. In: Proc Symp Plant Tiss Cult. Science Press, Beijing, pp 43–50Google Scholar
  7. Foroughi-Wehr B, Friedt W (1984) Rapid production of recombinant barley yellow mosaic virus resistant Hordeum vulgare lines by anther culture. Theor Appl Genet 67:377–382Google Scholar
  8. Genovesi AD, Magill CW (1979) Improved rate of callus and green plant production from rice anther culture following cold shock. Crop Sci 19:662–664Google Scholar
  9. Glaszmann JC (1987) Isozymes and classification of Asian rice varieties. Theor Appl Genet 74:21–30Google Scholar
  10. Glaszmann JC, de los Reyes BG, Khush GS (1988) Electrophoretic variation of isozymes in plumules of rice (Oryza sativa L.) — a key to the identification of 76 alleles at 24 loci. IRRI Res Pap Ser 134Google Scholar
  11. Guiderdoni E, Vergara G, de los Reyes BG (1988) Segregation of 8 isozymes genes among microspore-derived calli of a japonica/indica cross of rice (Oryza sativa L.). Philipp J Crop Sci 13:65–68Google Scholar
  12. Guiderdoni E, Courtois B, Glaszmann JC (1989a) Use of isozyme markers to monitor recombination and assess gametic selection in anther culture derivatives of remote crosses of rice (Oryza sativa L.). In: Sitch LAS, Mujeeb-Kazi A (eds) Review of advances in plant biotechnology, 1985–1988. CIMMYT, Mexico D.F. and IRRI, Manila, pp 43–55Google Scholar
  13. Guiderdoni E, Glaszmann JC, Courtois B (1989b) Segregation of 12 isozyme genes among doubled haploid lines derived from a japonica/indica cross of rice (Oryza saltiva L.). Euphytica 42:45–53Google Scholar
  14. Hayward MD, Olesen A, Due IK, Jenkins R, Morris P (1990) Segregation of isozyme marker loci amongst androgenetic plants of Lolium perenne L. Plant Breed 104:68–71Google Scholar
  15. Ikehashi M, Araki H (1986) Genetics of F1 sterility in remote crosses of rice. In. Rice Genetics. IRRI, Manila, pp 119–130Google Scholar
  16. Kao KN, Wetter LR, Dyck J, Horn D, Ye CM, Harvey BL (1983) Inheritance of certain genetic traits in pollen plants from F1 hybrid barley. Genet Soc Can Bull 14Google Scholar
  17. McCouch SR, Kochert G, Yu ZH, Wang ZY, Khush GS, Coffman WR, Tanksley SD (1988) Molecular mapping of rice chromosomes. Theor Appl Genet 76:815–819Google Scholar
  18. Murashige T, Skoog F (1962) A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol Plant 15:473–497Google Scholar
  19. Nakagahra M (1986) Geographic distribution of gametophyte genes in wide crosses of rice cultivars. In: Rice genetics. IRRI, Manila, pp 73–82Google Scholar
  20. Oka HI (1988) Indica-japonica differentiation of rice cultivars. In: Origin of cultivated rice. Japan Sci Soc Press, Tokyo and Elsevier, pp 141–179Google Scholar
  21. Orton TJ, Browers MA (1985) Segregation of genetic markers among plants regenerated from cultured anthers of broccoli (Brassica oleracea var ‘italica’). Theor Appl Genet 69:637–643Google Scholar
  22. Pham JL, Glaszmann JC, Sano R, Barbier P, Ghesquière A, Second G (1990) Isozyme markers in rice: genetic analysis and linkage relationships. Genome 33:348–359Google Scholar
  23. Powell W, Borrino EM, Allison MJ, Griffiths DW, Asher MJC, Dunwell JM (1986) Genetical analysis of microspore derived plants of barley (Hordeum vulgare). Theor Appl Genet 72:619–626Google Scholar
  24. Raquin C (1982) Etude des ségrégations de gènes impliqués dans la pigmentation florale des pétunias issus d'androgenèse. CR Acad Sci Paris 294:335–338Google Scholar
  25. Rivard SR, Cappadocia M, Vincent G, Brisson N, Landry BS (1989) Restriction fragment length polymorphism (RFLP) analyses of plants produced by in vitro anther culture of Solarium chacoense Bitt. Theor Appl Genet 78:49–56Google Scholar
  26. Sato Y, Ishikawa R, Morishima H (1986) Gametic selection under different conditions in rice. Annu Rep Natl Inst Genet, Misima, pp 93–94Google Scholar
  27. Second G (1982) Origin of the genetic diversity of cultivated rice (Oryza sp.); study of the polymorphism scored at 40 isozyme loci. Jpn J Genet 57:35–57Google Scholar
  28. Shen TT, Hsueh CC, Liu C (1978) Genetic behavior of pollen plants in paddy rice [in Chinese]. In: Proc Symp Anther Culture. Science Press, Beijing, pp 255–257Google Scholar
  29. Siva Reddy V, Leelavathi S, Sen SK (1989) In vitro segregation of marker genes in anther cultures of rice. In Vitro Cell Dev Biol 23:515–518Google Scholar
  30. Wu KS, Glaszmann JC, Khush GS (1988) Chromosomal location of ten isozyme loci in rice (Oryza saltiva L.) through trisomic analysis. Biochem Genet 26:303–320Google Scholar

Copyright information

© Springer-Verlag 1991

Authors and Affiliations

  • E. Guiderdoni
    • 1
  1. 1.Plant Breeding DepartmentInternational Rice Research InstituteManilaPhilippines

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