N-methyl-D-aspartate-evoked calcium uptake by kitten visual cortex maintained in vitro
- Cite this article as:
- Feldman, D., Sherin, J.E., Press, W.A. et al. Exp Brain Res (1990) 80: 252. doi:10.1007/BF00228153
- 34 Downloads
As a functional measure of NMDA receptor effectiveness in kitten striate cortex, the uptake of 45Ca by visual cortical slices was measured after 2 minute bath applications of N-methyl-d-aspartate (NMDA). Significant Ca uptake occured in response to 12.5–100 μM NMDA in slices prepared from visual cortex of normal animals aged 28–48 days. Basal uptake (in the absence of NMDA) was increased and evoked uptake was decreased in visual cortical slices prepared from age-matched dark-reared animals. Four days of binocular deprivation in otherwise normally reared animals had no effect on basal uptake, but significantly lowered NMDAevoked Ca uptake at agonist concentrations greater than 25 μM. These data suggest that even brief manipulations of sensory experience are sufficient to alter visual cortical calcium regulation.