Acta Neuropathologica

, Volume 84, Issue 3, pp 265–272

Monoclonal antibodies with selective specificity for Alzheimer Tau are directed against phosphatase-sensitive epitopes

  • Marc Mercken
  • Marc Vandermeeren
  • Ursula Lübke
  • Jan Six
  • Jef Boons
  • André Van de Voorde
  • Jean-Jacques Martin
  • Jan Gheuens
Regular Papers

Summary

A modified form of the microtubule-associated protein Tau is the major component of the paired helical filaments (PHF) found in Alzheimer's disease. The characterization of these posttranslational Tau modifications is hindered by the lack of sufficient PHF-Tau-specific markers. Here we describe several monoclonal antibodies, prepared by immunization with PHF, two of which showed a selective specificity for PHF-Tau without cross-reactivity with normal Tau. Epitope recognition by these two monoclonals was sensitive to alkaline phosphatase treatment. In Western blotting these monoclonal antibodies reacted specifically with the abnormally phosphorylated epitopes on Alzheimer's disease-associated PHF-Tau. One of the new antibodies can be used for the construction of a sandwich enzyme-linked immunosorbent assay for the specific detection of PHF-Tau without cross-reactivity to normal Tau proteins.

Key words

Alzheimer's disease Tau Phosphorylation Monoclonal antibodies 

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Copyright information

© Springer-Verlag 1992

Authors and Affiliations

  • Marc Mercken
    • 1
    • 2
  • Marc Vandermeeren
    • 1
    • 2
  • Ursula Lübke
    • 3
  • Jan Six
    • 1
    • 2
  • Jef Boons
    • 2
  • André Van de Voorde
    • 1
  • Jean-Jacques Martin
    • 3
  • Jan Gheuens
    • 2
  1. 1.Innogenetics, Industriepark ZwijnaardeGentBelgium
  2. 2.Laboratorie of Neurobiology Born-Bunge FoundationUniversity of Antwerp (UIA)WilrijkBelgium
  3. 3.Laboratorie of Neuropathology, Born-Bunge FoundationUniversity of Antwerp (UIA)WilrijkBelgium
  4. 4.Laboratory for Molecular Neuroscience and Aging Research, Mailman Research CenterMcLean HospitalBelmontUSA
  5. 5.Janssen Research FoundationBeerseBelgium

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