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Acta Neuropathologica

, Volume 84, Issue 6, pp 606–612 | Cite as

Expression of leukocyte antigen CD34 by brain capillaries in Alzheimer's disease and neurologically normal subjects

  • Rajesh N. Kalaria
  • Stephanie N. Kroon
Regular Papers

Summary

We studied the expression of a hemopoietic progenitor cell antigen, designated CD34, in brains from subjects with Alzheimer's disease (AD) and nonneurological controls. Immunoblots of brain microvessel proteins probed with monoclonal antibody QBEND/10 to the leukocyte antigen CD34 recognized a protein band with an apparent molecular mass of 90–100 kDa. Immunocytochemical staining of brain tissue sections showed CD34 to be expressed by all microvasculature including those of the circumventricular organs. In normal control brains such specific staining exhibited by QBEND/10 was indistinguishable from that obtained with collagen IV antibodies. In AD, however, increased vascular tendrils in form of endothelial abluminal processes and intraparenchymal abnormalities were evident in cortical and hippocampal regions, predominant in cases with severe pathology. Our results demonstrate that the leukocyte antigen CD34 is localized with the vascular endothelium throughout the human brain. These results also suggest that CD34 detects endothelial abnormalities in brains of AD subjects and support previous observations on the usefulness of CD34 to label abluminal microprocesses.

Key words

Alzheimer's disease Brain endothelium Blood-brain barrier Hemopoietic progenitor cells Leukocyte antigen CD34 

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Copyright information

© Springer-Verlag 1992

Authors and Affiliations

  • Rajesh N. Kalaria
    • 1
    • 2
  • Stephanie N. Kroon
    • 1
    • 2
  1. 1.Department of NeurologyCase Western Reserve University School of MedicineClevelandUSA
  2. 2.Department of NeurosciencesCase Western Reserve University School of MedicineClevelandUSA

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